PMID- 20018805
OWN - NLM
STAT- MEDLINE
DCOM- 20100204
LR  - 20231213
IS  - 1541-6100 (Electronic)
IS  - 0022-3166 (Linking)
VI  - 140
IP  - 2
DP  - 2010 Feb
TI  - Hepatic acyl-coenzyme a:cholesterol acyltransferase-2 expression is decreased in 
      mice with hyperhomocysteinemia.
PG  - 231-7
LID - 10.3945/jn.109.112920 [doi]
AB  - Alterations in lipid metabolism may contribute to the pathology of 
      hyperhomocysteinemia (HHcy). Our objective in this study was to test the 
      hypothesis that HHcy is associated with changes in liver acyl CoA:cholesterol 
      acyl transferase 2 (ACAT2) expression and cholesteryl esters (CE) in mice with 
      HHcy. ACAT2 is encoded by Soat2 and functions to catalyze the esterification of 
      cholesterol with acyl-CoA. Mice heterozygous for disruption of the 
      cystathionine-beta-synthase gene (Cbs +/-) and C57BL/6 mice (Cbs +/+) were fed a 
      control diet or a diet high in l-methionine (8.60 g/kg) and low in folic acid 
      (0.20 mg/kg) to induce HHcy (HH diet). Lower Soat2 mRNA (P < 0.05) and ACAT 
      protein (P < 0.001), higher total oleic acid [18:1(n-9)], and lower CE 18:1(n-9) 
      was found in liver from Cbs +/- mice fed the HH diet, with higher plasma total 
      homocysteine concentrations, than Cbs +/+ mice fed the control diet (35.01 +/- 
      5.6 vs. 2.21 +/- 0.6 mumol/L, respectively). In silico searches identified a 
      CpG-rich region in the 5' portion of the Soat2 gene, which was differentially 
      methylated (P < 0.05) in Cbs +/- mice fed the HH diet than in Cbs +/+ mice fed 
      the control diet and was accompanied by higher (P < 0.05) B1 repeat element 
      methylation, an indicator of global de novo methylation. These findings show 
      altered methylation and expression of Soat2/ACAT2 in liver from mice with HHcy 
      and suggest a role for changes in liver CE in the pathology of HHcy.
FAU - Devlin, Angela M
AU  - Devlin AM
AD  - Department of Pathology and Laboratory Medicine, Pediatrics, Child and Family 
      Research Institute, University of British Columbia, Vancouver V5Z 4H4, Canada. 
      angela.devlin@ubc.ca
FAU - Singh, Ranji
AU  - Singh R
FAU - Bottiglieri, Teodoro
AU  - Bottiglieri T
FAU - Innis, Sheila M
AU  - Innis SM
FAU - Green, Tim J
AU  - Green TJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091216
PL  - United States
TA  - J Nutr
JT  - The Journal of nutrition
JID - 0404243
RN  - 0 (Acyl Coenzyme A)
RN  - 0 (Cholesterol Esters)
RN  - 0 (RNA, Messenger)
RN  - 0LVT1QZ0BA (Homocysteine)
RN  - 2UMI9U37CP (Oleic Acid)
RN  - AE28F7PNPL (Methionine)
RN  - EC 2.3.1.26 (Sterol O-Acyltransferase)
RN  - EC 4.2.1.22 (Cystathionine beta-Synthase)
SB  - IM
MH  - Acyl Coenzyme A/*metabolism
MH  - Animals
MH  - Cholesterol Esters/*metabolism
MH  - CpG Islands
MH  - Cystathionine beta-Synthase/genetics/metabolism
MH  - Diet
MH  - Folic Acid Deficiency/metabolism
MH  - Homocysteine/*blood
MH  - Hyperhomocysteinemia/blood/genetics/*metabolism
MH  - Lipid Metabolism
MH  - Liver/*metabolism
MH  - Methionine/administration & dosage/metabolism/pharmacology
MH  - Methylation
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Oleic Acid/*metabolism
MH  - RNA, Messenger/metabolism
MH  - Sterol O-Acyltransferase/genetics/*metabolism
MH  - Sterol O-Acyltransferase 2
EDAT- 2009/12/19 06:00
MHDA- 2010/02/05 06:00
CRDT- 2009/12/19 06:00
PHST- 2009/12/19 06:00 [entrez]
PHST- 2009/12/19 06:00 [pubmed]
PHST- 2010/02/05 06:00 [medline]
AID - S0022-3166(22)06953-X [pii]
AID - 10.3945/jn.109.112920 [doi]
PST - ppublish
SO  - J Nutr. 2010 Feb;140(2):231-7. doi: 10.3945/jn.109.112920. Epub 2009 Dec 16.