PMID- 2002028
OWN - NLM
STAT- MEDLINE
DCOM- 19910417
LR  - 20210210
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 266
IP  - 8
DP  - 1991 Mar 15
TI  - Ligand-binding characteristics of rat serum-type mannose-binding protein (MBP-A). 
      Homology of binding site architecture with mammalian and chicken hepatic lectins.
PG  - 4810-5
AB  - Sugar-binding characteristics of rat serum mannose-binding protein (MBP) were 
      studied using the carbohydrate-recognition domain of this protein expressed from 
      a cloned cDNA. To assess the binding affinity of various test compounds, they 
      were added as inhibitors in a binding assay in which 125I-MBP was incubated with 
      yeast cells and the extent of binding was estimated from the radioactivity 
      associated with the pelleted cells. The results of such inhibition assays suggest 
      that MBP has a small binding site which is probably of the trough-type. The 3- 
      and 4-OH of the target sugar are indispensable, while the 6-OH is not required. 
      These characteristics are shared by the rat hepatic lectin and chicken hepatic 
      lectin, both of which are C-type lectins containing carbohydrate-recognition 
      domains highly homologous to that of MBP. Apparently, the related primary 
      structures of these lectins give rise to similar gross architecture of their 
      binding sites, despite the fact that each exhibits different sugar binding 
      specificities.
FAU - Lee, R T
AU  - Lee RT
AD  - Department of Biology, Johns Hopkins University, Baltimore, Maryland 21218.
FAU - Ichikawa, Y
AU  - Ichikawa Y
FAU - Fay, M
AU  - Fay M
FAU - Drickamer, K
AU  - Drickamer K
FAU - Shao, M C
AU  - Shao MC
FAU - Lee, Y C
AU  - Lee YC
LA  - eng
GR  - DK09970/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Carrier Proteins)
RN  - 0 (Lectins)
RN  - 0 (Ligands)
RN  - 0 (Mannose-Binding Lectins)
SB  - IM
MH  - Animals
MH  - Carrier Proteins/*metabolism
MH  - Chickens
MH  - Lectins/*metabolism
MH  - Ligands
MH  - Liver/*metabolism
MH  - Mannose-Binding Lectins
MH  - Rats
MH  - Sequence Homology, Nucleic Acid
MH  - Substrate Specificity
EDAT- 1991/03/15 00:00
MHDA- 1991/03/15 00:01
CRDT- 1991/03/15 00:00
PHST- 1991/03/15 00:00 [pubmed]
PHST- 1991/03/15 00:01 [medline]
PHST- 1991/03/15 00:00 [entrez]
AID - S0021-9258(19)67721-5 [pii]
PST - ppublish
SO  - J Biol Chem. 1991 Mar 15;266(8):4810-5.