PMID- 20020886
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20121002
LR  - 20091221
IS  - 1537-6524 (Electronic)
IS  - 1537-6516 (Linking)
VI  - 18
IP  - 1
DP  - 2008
TI  - Increased Antioxidant Potential and Decreased Free Radical Production in Response 
      to Mild Injection of Crude Venom, Cerastes cerastes gasperetti.
PG  - 11-6
LID - 10.1080/15376510701728455 [doi]
AB  - ABSTRACT The purpose of this study was to examine the effects of Cerastes 
      cerastes gasperetti crude venom (CCV) on free radical (FR) production and 
      biological antioxidant potential (BAP). Thirty male rats, matched with age and 
      weight, were divided randomly into three groups: control (C), negative control 
      (NC), and experimental (E). Animals in the control group served as control for 
      needlestick and venom injection. Animals in the negative control group, which 
      served as control for venom-induced effects, were injected intraperitoneally (IP) 
      with saline for 8 days, whereas animals of the experimental group were injected 
      IP with crude CCV, at a dose of 1/4 LD(50), for the same period. All animals of 
      the three groups remained under normal laboratory and dietary conditions. Blood 
      samples were collected by heart puncture technique and were analyzed immediately 
      for FRs and BAP. ANOVA analysis indicated a significant difference (p < 0.05) 
      among group means. Tukey-Kramer multiple comparisons showed that FRs increased 
      significantly (p < 0.05) in the NC group, implying that needlestick during 
      injection of saline for 8 days caused internal organ injury sufficient enough to 
      induce reactive oxygen species (ROS) generation. Surprisingly, there were no 
      significant (p > 0.05) changes in FRs in the E group as compared with the C 
      group, which indicates that CCV counteracted the effects induced by ROS. BAP 
      increased significantly (p < 0.05) in the E group as compared with the NC group, 
      reflecting the activation of some antioxidant enzymes. Linear regression of FRs 
      on BAP was significant (p < 0.05) in both the C and E groups. Based on the 
      results of this study, it can be concluded that the CCV contains biochemical 
      factors that activate antioxidant enzymes, as evident by elevation in the BAP 
      that depresses ROS formation.
FAU - Haffor, A S A
AU  - Haffor AS
AD  - Department of Zoology, College of Science, P.O. Box 2455, King Saud University, 
      Riyadh, Saudi Arabia, 11451.
FAU - Al-Sadoon, M K
AU  - Al-Sadoon MK
LA  - eng
PT  - Journal Article
PL  - England
TA  - Toxicol Mech Methods
JT  - Toxicology mechanisms and methods
JID - 101134521
EDAT- 2008/01/01 00:00
MHDA- 2008/01/01 00:01
CRDT- 2009/12/22 06:00
PHST- 2009/12/22 06:00 [entrez]
PHST- 2008/01/01 00:00 [pubmed]
PHST- 2008/01/01 00:01 [medline]
AID - 10.1080/15376510701728455 [doi]
PST - ppublish
SO  - Toxicol Mech Methods. 2008;18(1):11-6. doi: 10.1080/15376510701728455.