PMID- 20021662
OWN - NLM
STAT- MEDLINE
DCOM- 20100212
LR  - 20211020
IS  - 1471-2202 (Electronic)
IS  - 1471-2202 (Linking)
VI  - 10
DP  - 2009 Dec 18
TI  - Developmental iodine deficiency resulting in hypothyroidism reduces hippocampal 
      ERK1/2 and CREB in lactational and adolescent rats.
PG  - 149
LID - 10.1186/1471-2202-10-149 [doi]
AB  - BACKGROUND: Developmental iodine deficiency (ID) leads to inadequate thyroid 
      hormone that impairs learning and memory with an unclear mechanism. Here, we show 
      that hippocampal extracellular signal-regulated kinase (ERK1/2) and cAMP response 
      element-binding protein (CREB) are implicated in the impaired learning and memory 
      in lactational and adolescent rat hippocampus following developmental ID and 
      hypothyroidism. METHODS: Three developmental rat models were created by 
      administrating dam rats with either iodine-deficient diet or propylthiouracil 
      (PTU, 5 ppm or 15 ppm)-added drinking water from gestational day (GD) 6 till 
      postnatal day (PN) 28. Then, the total and phorsporylated ERK1/2 and total and 
      phorsporylated CREB in the hippocampus were detected with western blot on PN14, 
      PN21, PN28 and PN42. RESULTS: The iodine-deficient and hypothyroid pups showed 
      lower serum FT3 and FT4 levels, smaller body size, and delayed eyes opening. The 
      mean number of surviving cells in the hippocampus of the iodine-deficient and 15 
      ppm PTU-treated rats was significantly reduced compared to controls (P < 0.05). 
      Iodine-deficient and 15 ppm PTU-treatment groups demonstrated significantly lower 
      level of total and phosphorylated ERK1/2 and CREB than the controls on PN14, PN21 
      and PN28 (P < 0.05, respectively). The reduction of ERK1/2 and CREB was not 
      reversible with the restoration of serum thyroid hormone concentrations on PN42. 
      CONCLUSIONS: Developmental ID and hypothyroidism down-regulate hippocampal ERK1/2 
      and CREB in lactational and adolescent rats.
FAU - Dong, Jing
AU  - Dong J
AD  - Department of Occupational and Environmental Health, School of Public Health, 
      China Medical University, Shenyang, PR China. dj814@163.com
FAU - Liu, Wanyang
AU  - Liu W
FAU - Wang, Yi
AU  - Wang Y
FAU - Hou, Yi
AU  - Hou Y
FAU - Xi, Qi
AU  - Xi Q
FAU - Chen, Jie
AU  - Chen J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091218
PL  - England
TA  - BMC Neurosci
JT  - BMC neuroscience
JID - 100966986
RN  - 9679TC07X4 (Iodine)
RN  - E0399OZS9N (Cyclic AMP)
RN  - EC 2.3.1.48 (CREB-Binding Protein)
RN  - EC 2.3.1.48 (Crebbp protein, rat)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 3)
SB  - IM
MH  - Animals
MH  - CREB-Binding Protein/*metabolism
MH  - Cyclic AMP/metabolism
MH  - Disease Models, Animal
MH  - Down-Regulation
MH  - Hippocampus/*growth & development/metabolism/pathology
MH  - Hypothyroidism/pathology/*physiopathology
MH  - Iodine/*deficiency
MH  - Lactation/metabolism
MH  - Learning Disabilities/*metabolism
MH  - Memory Disorders/*metabolism
MH  - Mitogen-Activated Protein Kinase 3/*metabolism
MH  - Neurons/metabolism/pathology
MH  - Random Allocation
MH  - Rats
MH  - Rats, Wistar
PMC - PMC2804698
EDAT- 2009/12/22 06:00
MHDA- 2010/02/13 06:00
PMCR- 2009/12/18
CRDT- 2009/12/22 06:00
PHST- 2009/09/29 00:00 [received]
PHST- 2009/12/18 00:00 [accepted]
PHST- 2009/12/22 06:00 [entrez]
PHST- 2009/12/22 06:00 [pubmed]
PHST- 2010/02/13 06:00 [medline]
PHST- 2009/12/18 00:00 [pmc-release]
AID - 1471-2202-10-149 [pii]
AID - 10.1186/1471-2202-10-149 [doi]
PST - epublish
SO  - BMC Neurosci. 2009 Dec 18;10:149. doi: 10.1186/1471-2202-10-149.