PMID- 2002282
OWN - NLM
STAT- MEDLINE
DCOM- 19910418
LR  - 20190516
IS  - 0741-5400 (Print)
IS  - 0741-5400 (Linking)
VI  - 49
IP  - 4
DP  - 1991 Apr
TI  - Effect of soluble aminated beta-1,3-D-polyglucose on human monocytes: stimulation 
      of cytokine and prostaglandin E2 production but not antigen-presenting function.
PG  - 342-51
AB  - Glucans are insoluble polymers of beta-1,3-linked glucose derived from yeast cell 
      walls that effectively activate macrophages. Recently, aminated derivatives of 
      beta-1,3-D-polyglucose have been developed that are soluble but also activate 
      murine macrophages. The current studies were undertaken to determine whether 
      soluble aminated beta-1,3-D-polyglucose (AG) would also stimulate human 
      monocytes. The AG employed contained less than 2 ng endotoxin/mg. AG induced the 
      production of intracellular, membrane-associated, and secreted forms of 
      interleukin 1 (IL1) in a dose-dependent manner, with 50 micrograms/ml yielding 
      maximal responses. AG also induced tumor necrosis factor-alpha (TNF alpha) 
      secretion by human monocytes. Prostaglandin E2 (PGE2) production was also 
      stimulated in a concentration-dependent manner. Quantitatively, optimal 
      stimulatory concentrations of AG were comparable to endotoxin in the capacity to 
      induce production of these various mediators. In contrast to its capacity to 
      induce production of IL1, TNF alpha, and PGE2, AG did not stimulate monocytes to 
      become more effective antigen presenting cells. These results indicate that AG is 
      potent inducer of proinflammatory mediators from human monocytes but does not 
      enhance their capacity to initiate immune responses.
FAU - Doita, M
AU  - Doita M
AD  - Harold C. Simmons Arthritis Research Center, University of Texas Southwestern 
      Medical School, Dallas.
FAU - Rasmussen, L T
AU  - Rasmussen LT
FAU - Seljelid, R
AU  - Seljelid R
FAU - Lipsky, P E
AU  - Lipsky PE
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Leukoc Biol
JT  - Journal of leukocyte biology
JID - 8405628
RN  - 0 (Cytokines)
RN  - 0 (Glucans)
RN  - 0 (HLA Antigens)
RN  - 0 (Interleukin-1)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (beta-Glucans)
RN  - 9051-97-2 (beta-1,3-glucan)
RN  - K7Q1JQR04M (Dinoprostone)
RN  - XXE1CET956 (Indomethacin)
SB  - IM
MH  - Cells, Cultured
MH  - Cytokines/*biosynthesis
MH  - Dinoprostone/*biosynthesis
MH  - Dose-Response Relationship, Drug
MH  - Flow Cytometry
MH  - Gene Expression Regulation
MH  - Glucans/*pharmacology
MH  - HLA Antigens/biosynthesis
MH  - Humans
MH  - In Vitro Techniques
MH  - Indomethacin/pharmacology
MH  - Interleukin-1/biosynthesis
MH  - Lipopolysaccharides/pharmacology
MH  - Monocytes/*metabolism
MH  - Tumor Necrosis Factor-alpha/biosynthesis
MH  - *beta-Glucans
EDAT- 1991/04/01 00:00
MHDA- 1991/04/01 00:01
CRDT- 1991/04/01 00:00
PHST- 1991/04/01 00:00 [pubmed]
PHST- 1991/04/01 00:01 [medline]
PHST- 1991/04/01 00:00 [entrez]
AID - 10.1002/jlb.49.4.342 [doi]
PST - ppublish
SO  - J Leukoc Biol. 1991 Apr;49(4):342-51. doi: 10.1002/jlb.49.4.342.