PMID- 20023267
OWN - NLM
STAT- MEDLINE
DCOM- 20100112
LR  - 20191210
IS  - 1943-7722 (Electronic)
IS  - 0002-9173 (Linking)
VI  - 133
IP  - 1
DP  - 2010 Jan
TI  - Diagnostic usefulness of fluorescent cytogenetics in differentiating chromophobe 
      renal cell carcinoma from renal oncocytoma: a validation study combining 
      metaphase and interphase analyses.
PG  - 116-26
LID - 10.1309/AJCPSATJTKBI6J4N [doi]
AB  - We investigated the usefulness of interphase fluorescence in situ hybridization 
      (FISH) analysis to differentiate between 11 chromophobe renal carcinomas and 12 
      renal oncocytomas, showing different clinical outcomes, when compared with 
      conventional metaphase cytogenetics by karyotyping. Karyotypically, 3 chromophobe 
      renal cell carcinomas showed losses of chromosomes, 3 were polyploid, 1 was 
      normal, and 4 failed to grow. Of 12 oncocytomas, 5 showed a normal numeric 
      karyotype and 6 additional structural rearrangements. FISH on chromophobe renal 
      cell carcinomas showed a high percentage of cases (10/11 [91%]) with multiple 
      numeric losses among chromosomes 1, 2, 6, 10, and 17; this interphase pattern was 
      observed irrespective of the 3 different metaphase karyotypes. Of 12 oncocytomas, 
      11 (92%) revealed a normal numeric chromosomal status showing at least 2 
      chromosomes without aneusomy by interphase FISH. The study demonstrates that 
      indeed FISH performed on formalin-fixed, paraffin-embedded tissue can provide 
      clinically useful information more reliably than karyotyping of most of these 
      tumors.
FAU - Brunelli, Matteo
AU  - Brunelli M
AD  - 1Department of Pathology, University of Verona, Verona, Italy.
FAU - Delahunt, Brett
AU  - Delahunt B
FAU - Gobbo, Stefano
AU  - Gobbo S
FAU - Tardanico, Regina
AU  - Tardanico R
FAU - Eccher, Albino
AU  - Eccher A
FAU - Bersani, Samantha
AU  - Bersani S
FAU - Cossu-Rocca, Paolo
AU  - Cossu-Rocca P
FAU - Parolini, Claudia
AU  - Parolini C
FAU - Balzarini, Piera
AU  - Balzarini P
FAU - Menestrina, Fabio
AU  - Menestrina F
FAU - Cheng, Liang
AU  - Cheng L
FAU - Eble, John Nelson
AU  - Eble JN
FAU - Martignoni, Guido
AU  - Martignoni G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Validation Study
PL  - England
TA  - Am J Clin Pathol
JT  - American journal of clinical pathology
JID - 0370470
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - Adenoma, Oxyphilic/*diagnosis/genetics
MH  - Carcinoma, Renal Cell/*diagnosis/genetics
MH  - *Chromosome Aberrations
MH  - DNA, Neoplasm/analysis
MH  - Diagnosis, Differential
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Interphase/genetics
MH  - Kidney Neoplasms/*diagnosis/genetics
MH  - Metaphase/genetics
MH  - Tissue Array Analysis
EDAT- 2009/12/22 06:00
MHDA- 2010/01/13 06:00
CRDT- 2009/12/22 06:00
PHST- 2009/12/22 06:00 [entrez]
PHST- 2009/12/22 06:00 [pubmed]
PHST- 2010/01/13 06:00 [medline]
AID - 133/1/116 [pii]
AID - 10.1309/AJCPSATJTKBI6J4N [doi]
PST - ppublish
SO  - Am J Clin Pathol. 2010 Jan;133(1):116-26. doi: 10.1309/AJCPSATJTKBI6J4N.