PMID- 20025734
OWN - NLM
STAT- MEDLINE
DCOM- 20100315
LR  - 20211020
IS  - 1471-2407 (Electronic)
IS  - 1471-2407 (Linking)
VI  - 9
DP  - 2009 Dec 21
TI  - Array-based DNA methylation profiling of primary lymphomas of the central nervous 
      system.
PG  - 455
LID - 10.1186/1471-2407-9-455 [doi]
AB  - BACKGROUND: Although primary lymphomas of the central nervous system (PCNSL) and 
      extracerebral diffuse large B-cell lymphoma (DLBCL) cannot be distinguished 
      histologically, it is still a matter of debate whether PCNSL differ from systemic 
      DLBCL with respect to their molecular features and pathogenesis. Analysis of the 
      DNA methylation pattern might provide further data distinguishing these entities 
      at a molecular level. METHODS: Using an array-based technology we have assessed 
      the DNA methylation status of 1,505 individual CpG loci in five PCNSL and 
      compared the results to DNA methylation profiles of 49 DLBCL and ten 
      hematopoietic controls. RESULTS: We identified 194 genes differentially 
      methylated between PCNSL and normal controls. Interestingly, Polycomb target 
      genes and genes with promoters showing a high CpG content were significantly 
      enriched in the group of genes hypermethylated in PCNSL. However, PCNSL and 
      systemic DLBCL did not differ in their methylation pattern. CONCLUSIONS: Based on 
      the data presented here, PCNSL and DLBCL do not differ in their DNA methylation 
      pattern. Thus, DNA methylation analysis does not support a separation of PCNSL 
      and DLBCL into individual entities. However, PCNSL and DLBCL differ in their DNA 
      methylation pattern from non- malignant controls.
FAU - Richter, Julia
AU  - Richter J
AD  - Institute of Human Genetics, University Hospital Schleswig-Holstein Campus 
      Kiel/Christian-Albrechts University Kiel, Germany. jrichter@medgen.uni-kiel.de
FAU - Ammerpohl, Ole
AU  - Ammerpohl O
FAU - Martin-Subero, Jose I
AU  - Martin-Subero JI
FAU - Montesinos-Rongen, Manuel
AU  - Montesinos-Rongen M
FAU - Bibikova, Marina
AU  - Bibikova M
FAU - Wickham-Garcia, Eliza
AU  - Wickham-Garcia E
FAU - Wiestler, Otmar D
AU  - Wiestler OD
FAU - Deckert, Martina
AU  - Deckert M
FAU - Siebert, Reiner
AU  - Siebert R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091221
PL  - England
TA  - BMC Cancer
JT  - BMC cancer
JID - 100967800
RN  - 0 (Polycomb-Group Proteins)
RN  - 0 (Repressor Proteins)
SB  - IM
MH  - Central Nervous System Neoplasms/*genetics/metabolism/pathology
MH  - Cluster Analysis
MH  - CpG Islands/genetics
MH  - *DNA Methylation
MH  - Embryonic Stem Cells/metabolism
MH  - Female
MH  - Gene Expression Profiling/methods
MH  - Gene Expression Regulation, Neoplastic
MH  - Hematopoiesis/genetics
MH  - Humans
MH  - Lymphoma/*genetics
MH  - *Oligonucleotide Array Sequence Analysis/methods
MH  - Polycomb-Group Proteins
MH  - Repressor Proteins/metabolism/physiology
PMC - PMC2807878
EDAT- 2009/12/23 06:00
MHDA- 2010/03/17 06:00
PMCR- 2009/12/21
CRDT- 2009/12/23 06:00
PHST- 2009/07/16 00:00 [received]
PHST- 2009/12/21 00:00 [accepted]
PHST- 2009/12/23 06:00 [entrez]
PHST- 2009/12/23 06:00 [pubmed]
PHST- 2010/03/17 06:00 [medline]
PHST- 2009/12/21 00:00 [pmc-release]
AID - 1471-2407-9-455 [pii]
AID - 10.1186/1471-2407-9-455 [doi]
PST - epublish
SO  - BMC Cancer. 2009 Dec 21;9:455. doi: 10.1186/1471-2407-9-455.