PMID- 20030086
OWN - NLM
STAT- MEDLINE
DCOM- 20100112
LR  - 20191210
IS  - 1001-5302 (Print)
IS  - 1001-5302 (Linking)
VI  - 34
IP  - 18
DP  - 2009 Sep
TI  - [Rapid simultaneous determination of main nucleosides in Cordyceps sinensis with 
      LC-ESI-MS].
PG  - 2349-52
AB  - OBJECTIVE: To establish an LC-MS method for rapid simultaneous determination of 
      adenine,adenosine and cordycepin in Cordyceps sinensis. METHOD: An electrospray 
      ionization (ESI) interface and selective ion monitoring (SIM) mode were used. The 
      analytical column was a Shimadzu VP-ODS column (2.0 mm x 150 mm) and the mobile 
      phase was water-methanol-formic acid (92:7:1). Methanol was used as extraction 
      solvent and 2-chloroadenosine was used as internal standard for this assay. 
      RESULT: The regression equations and coefficient were Y = 0.07264X + 0.00622 and 
      r = 0.9987 for adenine, Y = 0.1597X + 0.0146 and r = 0.9991 for adenosine, Y = 
      0.1942X + 0.0186 and r = 0.9994 for cordycepin. The linear range was 0.8-130.0 mg 
      x L(-1), 0.5 - 124.5 mg x L(-1) and 0.5-128.5 mg x L(-1) for adenine, adenosine 
      and cordycepin, respectively. The average recovery was 98.76%, 99.37% and 99.26% 
      for adenine, adenosine and cordycepin, respectively. CONCLUSION: This established 
      method was highly sensitive, fast and selective, which can be used for rapid 
      simultaneous determination of adenine, adenosine and cordycepin in C. sinensis. 
      This method also can be applied for the quality control of C. sinensis.
FAU - Zhou, Jufeng
AU  - Zhou J
AD  - Department of Biochemistry, Xiangnan College, Chenzhou 423000, China.
FAU - Huang, Lanfang
AU  - Huang L
FAU - Guo, Fangqiu
AU  - Guo F
LA  - chi
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhongguo Zhong Yao Za Zhi
JT  - Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese 
      materia medica
JID - 8913656
RN  - 0 (Nucleosides)
SB  - IM
MH  - Animals
MH  - Chromatography, Liquid/*methods
MH  - Cordyceps/*chemistry
MH  - Moths/*chemistry
MH  - Nucleosides/*chemistry
MH  - Sensitivity and Specificity
MH  - Spectrometry, Mass, Electrospray Ionization/*methods
EDAT- 2009/12/25 06:00
MHDA- 2010/01/13 06:00
CRDT- 2009/12/25 06:00
PHST- 2009/12/25 06:00 [entrez]
PHST- 2009/12/25 06:00 [pubmed]
PHST- 2010/01/13 06:00 [medline]
PST - ppublish
SO  - Zhongguo Zhong Yao Za Zhi. 2009 Sep;34(18):2349-52.