PMID- 20030715
OWN - NLM
STAT- MEDLINE
DCOM- 20100823
LR  - 20240312
IS  - 1574-695X (Electronic)
IS  - 0928-8244 (Print)
IS  - 0928-8244 (Linking)
VI  - 58
IP  - 3
DP  - 2010 Apr
TI  - MMP-9/TIMP-1 imbalance induced in human dendritic cells by Porphyromonas 
      gingivalis.
PG  - 314-21
LID - 10.1111/j.1574-695X.2009.00637.x [doi]
AB  - Matrix metalloproteinase-9 (MMP-9) cleaves collagen, allowing leukocytes to 
      traffic toward the vasculature and the lymphatics. When MMP-9 is unregulated by 
      tissue inhibitor of metalloproteinase-1 (TIMP-1), this can lead to tissue 
      destruction. Dendritic cells (DCs) infiltrate the oral mucosa increasingly in 
      chronic periodontitis, characterized by infection with several pathogens 
      including Porphyromonas gingivalis. In this study, human monocyte-derived DCs 
      were pulsed with different doses of lipopolysaccharide of P. gingivalis 381 and 
      of Escherichia coli type strain 25922, as well as whole live isogenic 
      fimbriae-deficient mutant strains of P. gingivalis 381. Levels of induction of 
      MMP-9 and TIMP-1, as well as interleukin-10 (IL-10), which reportedly inhibits 
      MMP-9 induction, were measured by several approaches. Our results reveal that 
      lipopolysaccharide of P. gingivalis, compared with lipopolysaccharide from E. 
      coli type strain 25922, is a relatively potent inducer of MMP-9, but a weak 
      inducer of TIMP-1, contributing to a high MMP-9/TIMP-1 ratio.Whole live P. 
      gingivalis strain 381, major fimbriae mutant DPG-3 and double mutant MFB were 
      potent inducers of MMP-9, but minor fimbriae mutant MFI was not. MMP-9 induction 
      was inversely proportional to IL-10 induction. These results suggest that 
      lipopolysaccharide and the minor and the major fimbriae of P. gingivalis may play 
      distinct roles in induction by DCs of MMP-9, a potent mediator of local tissue 
      destruction and leukocyte trafficking.
FAU - Jotwani, Ravi
AU  - Jotwani R
AD  - Department of Periodontics and Implantology, Stony Brook University, NY 
      11794-8703, USA.
FAU - Eswaran, Sridhar V K
AU  - Eswaran SV
FAU - Moonga, Surinder
AU  - Moonga S
FAU - Cutler, Christopher W
AU  - Cutler CW
LA  - eng
GR  - R01 DE014328-07S1/DE/NIDCR NIH HHS/United States
GR  - R01 DE014328/DE/NIDCR NIH HHS/United States
GR  - R01 DE014328-03/DE/NIDCR NIH HHS/United States
GR  - R21 DE020916/DE/NIDCR NIH HHS/United States
GR  - R01 DE014328-01/DE/NIDCR NIH HHS/United States
GR  - R01 DE014328-06A1/DE/NIDCR NIH HHS/United States
GR  - R01 DE014328-07/DE/NIDCR NIH HHS/United States
GR  - R01 DE14328/DE/NIDCR NIH HHS/United States
GR  - R01 DE014328-02/DE/NIDCR NIH HHS/United States
GR  - R01 DE014328-05/DE/NIDCR NIH HHS/United States
GR  - R01 DE014328-08/DE/NIDCR NIH HHS/United States
GR  - R01 DE014328-04/DE/NIDCR NIH HHS/United States
GR  - R01 DE014328-09/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - FEMS Immunol Med Microbiol
JT  - FEMS immunology and medical microbiology
JID - 9315554
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Tissue Inhibitor of Metalloproteinase-1)
RN  - 130068-27-8 (Interleukin-10)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
SB  - IM
MH  - Cells, Cultured
MH  - Dendritic Cells/cytology/immunology
MH  - Escherichia coli/immunology/metabolism
MH  - Humans
MH  - Interleukin-10/immunology
MH  - Lipopolysaccharides/immunology
MH  - Matrix Metalloproteinase 9/*metabolism
MH  - Monocytes/cytology
MH  - Porphyromonas gingivalis/genetics/*immunology/metabolism
MH  - Tissue Inhibitor of Metalloproteinase-1/*metabolism
MH  - Up-Regulation
PMC - PMC2871062
MID - NIHMS162124
EDAT- 2009/12/25 06:00
MHDA- 2010/08/24 06:00
PMCR- 2010/07/01
CRDT- 2009/12/25 06:00
PHST- 2009/12/25 06:00 [entrez]
PHST- 2009/12/25 06:00 [pubmed]
PHST- 2010/08/24 06:00 [medline]
PHST- 2010/07/01 00:00 [pmc-release]
AID - FIM637 [pii]
AID - 10.1111/j.1574-695X.2009.00637.x [doi]
PST - ppublish
SO  - FEMS Immunol Med Microbiol. 2010 Apr;58(3):314-21. doi: 
      10.1111/j.1574-695X.2009.00637.x.