PMID- 20032174
OWN - NLM
STAT- MEDLINE
DCOM- 20100402
LR  - 20211020
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Print)
IS  - 0022-538X (Linking)
VI  - 84
IP  - 6
DP  - 2010 Mar
TI  - Identification of the CD163 protein domains involved in infection of the porcine 
      reproductive and respiratory syndrome virus.
PG  - 3101-5
LID - 10.1128/JVI.02093-09 [doi]
AB  - Scavenger receptor CD163 is a key entry mediator for porcine reproductive and 
      respiratory syndrome virus (PRRSV). To identify the CD163 protein domains 
      involved in PRRSV infection, deletion mutants and chimeric mutants were created. 
      Infection experiments revealed that scavenger receptor cysteine-rich (SRCR) 
      domain 5 (SRCR 5) is essential for PRRSV infection, while the four N-terminal 
      SRCR domains and the cytoplasmic tail are not required. The remaining CD163 
      protein domains need to be present but can be replaced by corresponding SRCR 
      domains from CD163-L1, resulting in reduced (SRCR 6 and interdomain regions) or 
      unchanged (SRCR 7 to SRCR 9) infection efficiency. In addition, CD163-specific 
      antibodies recognizing SRCR 5 are able to reduce PRRSV infection.
FAU - Van Gorp, Hanne
AU  - Van Gorp H
AD  - Department of Virology, Parasitology and Immunology, Ghent University, 9820 
      Merelbeke, Belgium.
FAU - Van Breedam, Wander
AU  - Van Breedam W
FAU - Van Doorsselaere, Jan
AU  - Van Doorsselaere J
FAU - Delputte, Peter L
AU  - Delputte PL
FAU - Nauwynck, Hans J
AU  - Nauwynck HJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091223
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation, Myelomonocytic)
RN  - 0 (CD163 antigen)
RN  - 0 (Protein Isoforms)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Scavenger Receptors, Class B)
SB  - IM
MH  - Animals
MH  - Antigens, CD/*chemistry/genetics/*metabolism
MH  - Antigens, Differentiation, Myelomonocytic/*chemistry/genetics/*metabolism
MH  - Humans
MH  - Porcine Reproductive and Respiratory Syndrome/*metabolism/virology
MH  - Porcine respiratory and reproductive syndrome 
      virus/genetics/*metabolism/pathogenicity
MH  - Protein Isoforms/chemistry/genetics/metabolism
MH  - Protein Structure, Tertiary
MH  - Receptors, Cell Surface/*chemistry/genetics/*metabolism
MH  - Recombinant Fusion Proteins/chemistry/genetics/metabolism
MH  - Scavenger Receptors, Class B/genetics/metabolism
MH  - Sequence Deletion
MH  - Swine
MH  - Virus Internalization
PMC - PMC2826032
EDAT- 2009/12/25 06:00
MHDA- 2010/04/03 06:00
PMCR- 2010/09/01
CRDT- 2009/12/25 06:00
PHST- 2009/12/25 06:00 [entrez]
PHST- 2009/12/25 06:00 [pubmed]
PHST- 2010/04/03 06:00 [medline]
PHST- 2010/09/01 00:00 [pmc-release]
AID - JVI.02093-09 [pii]
AID - 2093-09 [pii]
AID - 10.1128/JVI.02093-09 [doi]
PST - ppublish
SO  - J Virol. 2010 Mar;84(6):3101-5. doi: 10.1128/JVI.02093-09. Epub 2009 Dec 23.