PMID- 20033390
OWN - NLM
STAT- MEDLINE
DCOM- 20100602
LR  - 20211020
IS  - 1432-2242 (Electronic)
IS  - 0040-5752 (Linking)
VI  - 120
IP  - 6
DP  - 2010 Apr
TI  - Fine mapping of pepper trichome locus 1 controlling trichome formation in 
      Capsicum annuum L. CM334.
PG  - 1099-106
LID - 10.1007/s00122-009-1237-5 [doi]
AB  - Trichomes are present on nearly all land plants and protect plants against insect 
      herbivores, drought and UV radiation. The trichome-bearing phenotype is conferred 
      by the dominant allele of the pepper trichome locus 1 (Ptl1) in Capsicum annuum, 
      Mexican 'Criollo de Morelos-334' (CM334). A genetic analysis using simple 
      sequence repeats from pepper cDNA identified the HpmsE031 marker as tightly 
      linked to Ptl1 in 653 individuals of an F(2) population derived from a cross 
      between CM334 and Chilsungcho varieties. A bacterial artificial chromosome (BAC) 
      library from CM334 covering 12x of the genome was screened using the HpmsE031 SSR 
      marker as a probe and three BAC clones were identified. The Ptl1 region was 
      covered by one 80 kb BAC clone, TT1B7. Fluorescence in situ hybridization (FISH) 
      confirmed that TT1B7 localized to pepper chromosome 10. One co-dominant marker, 
      Tco, and one dominant marker, Tsca, were successfully developed from the TT1B7 
      BAC sequence. Tco mapped 0.33 cM up from Ptl1 and Tsca mapped 0.75 cM down from 
      Ptl1. Analysis of the BAC sequence predicts the presence of 14 open reading 
      frames including 60S ribosomal protein L21-like protein (Solanum demissum), 
      protein kinase 2 (Nicotiana tabacum), hypothetical proteins, and unnamed protein 
      products. These results will provide not only useful information for map-based 
      cloning of Ptl1 in Capsicum but also the starting points for analysis of R-gene 
      cluster inked with Ptl1.
FAU - Kim, Hyun Jung
AU  - Kim HJ
AD  - Department of Plant Sciences, Plant Genomics and Breeding Institute, Seoul 
      National University, Seoul, 151-921, South Korea.
FAU - Han, Jung-Heon
AU  - Han JH
FAU - Kwon, Jin-Kyung
AU  - Kwon JK
FAU - Park, Minkyu
AU  - Park M
FAU - Kim, Byung-Dong
AU  - Kim BD
FAU - Choi, Doil
AU  - Choi D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091224
PL  - Germany
TA  - Theor Appl Genet
JT  - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
JID - 0145600
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Base Sequence
MH  - Capsicum/anatomy & histology/*genetics/*growth & development
MH  - Chromosome Segregation/genetics
MH  - Chromosomes, Artificial, Bacterial/genetics
MH  - Chromosomes, Plant/genetics
MH  - Gene Library
MH  - Genetic Loci/*genetics
MH  - Genetic Markers
MH  - In Situ Hybridization, Fluorescence
MH  - Minisatellite Repeats/genetics
MH  - Molecular Sequence Data
MH  - Phenotype
MH  - Physical Chromosome Mapping/*methods
MH  - Plant Epidermis/*anatomy & histology/*genetics/growth & development
MH  - Plant Leaves/*genetics/ultrastructure
MH  - Reproducibility of Results
EDAT- 2009/12/25 06:00
MHDA- 2010/06/03 06:00
CRDT- 2009/12/25 06:00
PHST- 2009/08/06 00:00 [received]
PHST- 2009/11/30 00:00 [accepted]
PHST- 2009/12/25 06:00 [entrez]
PHST- 2009/12/25 06:00 [pubmed]
PHST- 2010/06/03 06:00 [medline]
AID - 10.1007/s00122-009-1237-5 [doi]
PST - ppublish
SO  - Theor Appl Genet. 2010 Apr;120(6):1099-106. doi: 10.1007/s00122-009-1237-5. Epub 
      2009 Dec 24.