PMID- 20035815
OWN - NLM
STAT- MEDLINE
DCOM- 20100907
LR  - 20111117
IS  - 1879-1166 (Electronic)
IS  - 0198-8859 (Linking)
VI  - 71
IP  - 3
DP  - 2010 Mar
TI  - Rapid genetic screening for major human leukocyte antigen risk haplotypes in 
      patients with type 1 diabetes from Northeastern Brazil.
PG  - 277-80
LID - 10.1016/j.humimm.2009.12.008 [doi]
AB  - The aim of this study was to identify in the Brazilian population the frequency 
      of human leukocyte antigen (HLA) DQ2.5 and DQ8 haplotypes conferring risk for 
      type 1 diabetes (T1D), and to validate a new genotyping method aimed at cost 
      reduction and automation. A total of 184 children and adolescents with T1D and 
      184 healthy individuals from Recife (northeastern Brazil) were analyzed using the 
      conventional polymerase chain reaction-sequence-specific primers HLA genotyping 
      and a newly described Tag-single-nucleotide polymorphism real-time polymerase 
      chain reaction. The Tag-single-nucleotide polymorphism-based HLA genotyping 
      method was successfully validated, proved to be robust, with limited cost and 
      thus could be successfully used for the identification of genetic susceptibility 
      for T1D in areas with limited financial resources. Our findings report for the 
      first time the distribution of DQ2.5 and DQ8 HLA risk haplotypes associated with 
      T1D in northeastern Brazil and evidence a major risk for developing T1D when the 
      heterozygous DQ2.5/DQ8 or the homozygous DQ2.5/DQ2.5 haplotypes are present.
CI  - (c) 2010 American Society for Histocompatibility and Immunogenetics. Published by 
      Elsevier Inc. All rights reserved.
FAU - Brandao, Lucas C
AU  - Brandao LC
AD  - Laboratorio de Imunopatologia Keizo Azami (LIKA), Federal University of 
      Pernambuco, Recife, Pernambuco, Brazil.
FAU - Vatta, Serena
AU  - Vatta S
FAU - Guimaraes, Rafael
AU  - Guimaraes R
FAU - Segat, Ludovica
AU  - Segat L
FAU - Araujo, Jaqueline
AU  - Araujo J
FAU - De Lima Filho, Jose L
AU  - De Lima Filho JL
FAU - Arraes, Luiz C
AU  - Arraes LC
FAU - Not, Tarcisio
AU  - Not T
FAU - Crovella, Sergio
AU  - Crovella S
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100107
PL  - United States
TA  - Hum Immunol
JT  - Human immunology
JID - 8010936
RN  - 0 (HLA-DQ Antigens)
RN  - 0 (HLA-DQ2 antigen)
RN  - 0 (HLA-DQ8 antigen)
SB  - IM
MH  - Adolescent
MH  - Brazil
MH  - Child
MH  - Child, Preschool
MH  - Diabetes Mellitus, Type 1/epidemiology/*genetics/immunology
MH  - Female
MH  - Gene Frequency
MH  - Genetic Association Studies
MH  - Genetic Predisposition to Disease
MH  - Genetic Testing/*methods
MH  - HLA-DQ Antigens/*analysis
MH  - Haplotypes
MH  - Histocompatibility Testing/methods
MH  - Humans
MH  - Infant
MH  - Male
MH  - Polymorphism, Single Nucleotide
MH  - Risk Factors
MH  - Young Adult
EDAT- 2009/12/29 06:00
MHDA- 2010/09/08 06:00
CRDT- 2009/12/29 06:00
PHST- 2009/09/11 00:00 [received]
PHST- 2009/12/01 00:00 [revised]
PHST- 2009/12/17 00:00 [accepted]
PHST- 2009/12/29 06:00 [entrez]
PHST- 2009/12/29 06:00 [pubmed]
PHST- 2010/09/08 06:00 [medline]
AID - S0198-8859(09)00662-4 [pii]
AID - 10.1016/j.humimm.2009.12.008 [doi]
PST - ppublish
SO  - Hum Immunol. 2010 Mar;71(3):277-80. doi: 10.1016/j.humimm.2009.12.008. Epub 2010 
      Jan 7.