PMID- 20036682
OWN - NLM
STAT- MEDLINE
DCOM- 20100609
LR  - 20161125
IS  - 1879-3150 (Electronic)
IS  - 0041-0101 (Linking)
VI  - 55
IP  - 6
DP  - 2010 Jun 1
TI  - Isolation and characterization of two new Lys49 PLA2s with heparin neutralizing 
      properties from Bothrops moojeni snake venom.
PG  - 1080-92
LID - 10.1016/j.toxicon.2009.12.007 [doi]
AB  - Among the proteins and peptides already characterized in Bothrops moojeni venom, 
      two novel phospholipases A(2) (PLA(2)) have been purified and fully sequenced by 
      ESI-MS/MS techniques. Both of them belong to the enzymatically non-active Lys49 
      variants of PLA(2). They consist of 122 amino acids and share a characteristic 
      sequence in their C-terminal region composed of clusters of basic amino acids 
      known to interact with heparin. Thus, as already established, heparin can be used 
      as an antidote to antagonize some myotoxic PLA(2)s from venoms of Bothrops genus. 
      The two PLA(2) variants were shown to interact in vitro with unfractionated 
      heparin (UFH) and low molecular weight heparin (LMWH), neutralizing their 
      anticoagulant properties. Although the influences of PLA(2)s from snake venoms on 
      the blood coagulation system are known, their use to antagonize the anticoagulant 
      effect of heparin in vitro or in vivo has never been proposed. These finding 
      recommend diagnostic and therapeutic applications, which are currently 
      investigated.
CI  - Copyright 2009 Elsevier Ltd. All rights reserved.
FAU - Perchuc, Anna Maria
AU  - Perchuc AM
AD  - DSM Nutritional Products AG Branch Pentapharm, Aesch (BL), Switzerland. 
      annaperchuc@gmail.com
FAU - Menin, Laure
AU  - Menin L
FAU - Favreau, Philippe
AU  - Favreau P
FAU - Buhler, Beatrice
AU  - Buhler B
FAU - Bulet, Philippe
AU  - Bulet P
FAU - Schoni, Reto
AU  - Schoni R
FAU - Wilmer, Marianne
AU  - Wilmer M
FAU - Ernst, Beat
AU  - Ernst B
FAU - Stocklin, Reto
AU  - Stocklin R
LA  - eng
PT  - Journal Article
DEP - 20091228
PL  - England
TA  - Toxicon
JT  - Toxicon : official journal of the International Society on Toxinology
JID - 1307333
RN  - 0 (Crotalid Venoms)
RN  - 0 (Heparin Antagonists)
RN  - 0 (Protein Isoforms)
RN  - EC 3.1.1.32 (Phospholipases A)
RN  - K3Z4F929H6 (Lysine)
SB  - IM
MH  - Animals
MH  - Blood Coagulation/drug effects
MH  - Blood Coagulation Tests
MH  - Bothrops/*physiology
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - Chromatography, Gel
MH  - Chromatography, High Pressure Liquid
MH  - Crotalid Venoms/*chemistry/pharmacology
MH  - Fibroblasts/drug effects/pathology
MH  - Heparin Antagonists/*chemistry/pharmacology
MH  - Humans
MH  - Lysine/chemistry
MH  - Phospholipases A/*chemistry/pharmacology
MH  - Protein Isoforms/chemistry
MH  - Spectrometry, Mass, Electrospray Ionization
MH  - Tandem Mass Spectrometry
EDAT- 2009/12/29 06:00
MHDA- 2010/06/10 06:00
CRDT- 2009/12/29 06:00
PHST- 2009/08/10 00:00 [received]
PHST- 2009/12/09 00:00 [revised]
PHST- 2009/12/10 00:00 [accepted]
PHST- 2009/12/29 06:00 [entrez]
PHST- 2009/12/29 06:00 [pubmed]
PHST- 2010/06/10 06:00 [medline]
AID - S0041-0101(09)00576-5 [pii]
AID - 10.1016/j.toxicon.2009.12.007 [doi]
PST - ppublish
SO  - Toxicon. 2010 Jun 1;55(6):1080-92. doi: 10.1016/j.toxicon.2009.12.007. Epub 2009 
      Dec 28.