PMID- 20039314
OWN - NLM
STAT- MEDLINE
DCOM- 20100812
LR  - 20220331
IS  - 1097-4644 (Electronic)
IS  - 0730-2312 (Linking)
VI  - 109
IP  - 4
DP  - 2010 Mar 1
TI  - Derivation of murine induced pluripotent stem cells (iPS) and assessment of their 
      differentiation toward osteogenic lineage.
PG  - 643-52
LID - 10.1002/jcb.22440 [doi]
AB  - Induced pluripotent stem cells (iPSCs) have generated hope and excitement because 
      of the potential they possess for generating patient-specific embryonic-like stem 
      cells (ESCs). Although many hurdles remain to be solved before the cells can be 
      applied clinically; studies directed toward understanding factors that control 
      differentiation of the cells toward various cell lineages are prerequisites for 
      their future application. In the present study, we generated murine iPSC and 
      assessed their differentiation toward osteogenic lineage. Murine tail tip 
      fibroblasts were reprogrammed into embryonic-like state by transduction with 
      defined factors (Oct3/4, Sox2, c-Myc, and klf4) carried in a retroviral vector. 
      The reprogrammed cells expressed ESC markers, gave rise to three germ layers as 
      demonstrated by teratoma formation and immunofluorescence staining. These data 
      confirmed that the reprogrammed cells exhibited ESC-like state. Treatment of 
      iPSCs-derived embryoid bodies (EBs) with transforming growth factor beta 1 
      (TGF-beta1) in the presence of retinoic acid enhanced generation of MSC-like 
      cells. The MSCs-like cells expressed putative makers associated with MSCs; the 
      cells deposited calcium in vitro when cultured in osteogenic medium. 
      Interestingly MSCs-like cells generated from iPSC directed EBs by treatment with 
      retinoic acid and TGF-beta1 deposited more calcium in vitro than cells derived 
      without TGF-beta1 treatment. Taken together, the data demonstrate that iPSC give 
      rise to MSCs-like state and that the cells have potential to differentiate toward 
      osteoblasts. In addition, brief treatment of iPSC-derived EBs with TGF-beta1 may 
      be an approach for directing iPSC toward MSC-like state.
CI  - (c) 2009 Wiley-Liss, Inc.
FAU - Li, Feng
AU  - Li F
AD  - Department of Orthopaedics and Rehabilitation, Division of Musculoskeletal 
      Sciences, Pennsylvania State University College of Medicine, Hershey, 
      Pennsylvania 17033, USA.
FAU - Bronson, Sarah
AU  - Bronson S
FAU - Niyibizi, Christopher
AU  - Niyibizi C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Cell Biochem
JT  - Journal of cellular biochemistry
JID - 8205768
RN  - 0 (Klf4 protein, mouse)
RN  - 0 (Kruppel-Like Factor 4)
RN  - 0 (Transforming Growth Factor beta1)
SB  - IM
MH  - Animals
MH  - Cell Culture Techniques
MH  - Cell Differentiation
MH  - *Cell Lineage
MH  - Embryonic Stem Cells/cytology
MH  - Fibroblasts/cytology
MH  - Induced Pluripotent Stem Cells/*cytology
MH  - Kruppel-Like Factor 4
MH  - Mice
MH  - *Osteogenesis
MH  - Transforming Growth Factor beta1/pharmacology
EDAT- 2009/12/30 06:00
MHDA- 2010/08/13 06:00
CRDT- 2009/12/30 06:00
PHST- 2009/12/30 06:00 [entrez]
PHST- 2009/12/30 06:00 [pubmed]
PHST- 2010/08/13 06:00 [medline]
AID - 10.1002/jcb.22440 [doi]
PST - ppublish
SO  - J Cell Biochem. 2010 Mar 1;109(4):643-52. doi: 10.1002/jcb.22440.