PMID- 20039440
OWN - NLM
STAT- MEDLINE
DCOM- 20101004
LR  - 20181201
IS  - 1520-6033 (Electronic)
IS  - 1520-6033 (Linking)
VI  - 26
IP  - 3
DP  - 2010 May-Jun
TI  - A cell leakproof PLGA-collagen hybrid scaffold for cartilage tissue engineering.
PG  - 819-26
LID - 10.1002/btpr.375 [doi]
AB  - A cell leakproof porous poly(DL-lactic-co-glycolic acid) (PLGA)-collagen hybrid 
      scaffold was prepared by wrapping the surfaces of a collagen sponge except the 
      top surface for cell seeding with a bi-layered PLGA mesh. The PLGA-collagen 
      hybrid scaffold had a structure consisting of a central collagen sponge formed 
      inside a bi-layered PLGA mesh cup. The hybrid scaffold showed high mechanical 
      strength. The cell seeding efficiency was 90.0% when human mesenchymal stem cells 
      (MSCs) were seeded in the hybrid scaffold. The central collagen sponge provided 
      enough space for cell loading and supported cell adhesion, while the bi-layered 
      PLGA mesh cup protected against cell leakage and provided high mechanical 
      strength for the collagen sponge to maintain its shape during cell culture. The 
      MSCs in the hybrid scaffolds showed round cell morphology after 4 weeks culture 
      in chondrogenic induction medium. Immunostaining demonstrated that type II 
      collagen and cartilaginous proteoglycan were detected in the extracellular 
      matrices. Gene expression analyses by real-time PCR showed that the genes 
      encoding type II collagen, aggrecan, and SOX9 were upregulated. These results 
      indicated that the MSCs differentiated and formed cartilage-like tissue when 
      being cultured in the cell leakproof PLGA-collagen hybrid scaffold. The cell 
      leakproof PLGA-collagen hybrid scaffolds should be useful for applications in 
      cartilage tissue engineering.
CI  - Copyright 2009 American Institute of Chemical Engineers
FAU - Kawazoe, Naoki
AU  - Kawazoe N
AD  - Biomaterials Center, National Institute for Materials Science, 1-1 Namiki, 
      Tsukuba, Ibaraki 305-0044, Japan.
FAU - Inoue, Chieko
AU  - Inoue C
FAU - Tateishi, Tetsuya
AU  - Tateishi T
FAU - Chen, Guoping
AU  - Chen G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biotechnol Prog
JT  - Biotechnology progress
JID - 8506292
RN  - 0 (Collagen Type I)
RN  - 0 (Collagen Type II)
RN  - 0 (Collagen Type X)
RN  - 0 (RNA, Messenger)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 33X04XA5AT (Lactic Acid)
SB  - IM
MH  - Analysis of Variance
MH  - Animals
MH  - Cattle
MH  - Cells, Cultured
MH  - Collagen Type I/*chemistry/genetics/metabolism
MH  - Collagen Type II/genetics/*metabolism
MH  - Collagen Type X/genetics/*metabolism
MH  - Gene Expression
MH  - Histocytochemistry
MH  - Humans
MH  - Lactic Acid/*chemistry
MH  - Materials Testing
MH  - Mesenchymal Stem Cells/cytology/metabolism
MH  - Microscopy, Electron, Scanning
MH  - Polyglycolic Acid/*chemistry
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
MH  - Polymerase Chain Reaction
MH  - RNA, Messenger/analysis
MH  - Tissue Engineering/methods
MH  - Tissue Scaffolds/*chemistry
EDAT- 2009/12/30 06:00
MHDA- 2010/10/05 06:00
CRDT- 2009/12/30 06:00
PHST- 2009/12/30 06:00 [entrez]
PHST- 2009/12/30 06:00 [pubmed]
PHST- 2010/10/05 06:00 [medline]
AID - 10.1002/btpr.375 [doi]
PST - ppublish
SO  - Biotechnol Prog. 2010 May-Jun;26(3):819-26. doi: 10.1002/btpr.375.