PMID- 20041102
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20110714
LR  - 20211020
IS  - 2005-0399 (Electronic)
IS  - 2005-0380 (Print)
IS  - 2005-0380 (Linking)
VI  - 20
IP  - 4
DP  - 2009 Dec
TI  - Epstein-Barr virus-transformation of B-cell lines in ovarian cancer patients: 
      feasibility of genomic storage for unlimited use.
PG  - 243-5
LID - 10.3802/jgo.2009.20.4.243 [doi]
AB  - OBJECTIVE: The aim of the current study is to test whether immortalized 
      B-lymphocyte cell line via Ebstein-Barr virus (EBV) transformation is feasible 
      and can be an unlimited source of genome wide study. METHODS: We obtained 
      peripheral whole blood from 5 ovarian cancer patients and immortalized the B-cell 
      lines using EBV transformation. The success rate was analyzed and the 
      bio-identity of the genome was performed using human leukocyte antigen (HLA) 
      identity test. RESULTS: EBV transformation was successful in all 5 cases (95% 
      confidence interval, 46.3% to 100%). After cryopreservation of EBV-transformed 
      B-cell lines and subsequent thawing, we observed that all cell lines were viable 
      and proliferative. To check bio-identity, HLA-A, B, and DR were tested between 
      the genome of the original samples and the transformed samples. The HLA typing 
      revealed that all observed HLA-A, B, and DR type was identical in 5 cases before 
      and after EBV-transformation. CONCLUSION: The current results suggest that 
      EBV-transformation of peripheral blood is an efficient tool in genome banking. 
      The EBV-transformed B-cell lines may be a valuable resource of genome in 
      multi-center translational research by the Korean Gynecologic Oncology Group.
FAU - Kong, Sun-Young
AU  - Kong SY
AD  - Hematologic Malignancy Branch & Department of Laboratory Medicine, Center for 
      Clinical Services, Goyang, Korea.
FAU - Kang, Sokbom
AU  - Kang S
LA  - eng
PT  - Journal Article
DEP - 20091228
PL  - Korea (South)
TA  - J Gynecol Oncol
JT  - Journal of gynecologic oncology
JID - 101483150
PMC - PMC2799024
OTO - NOTNLM
OT  - Cryopreservation
OT  - DNA storage
OT  - EBV transformation
OT  - Epidemiology
OT  - Genomics
EDAT- 2009/12/31 06:00
MHDA- 2009/12/31 06:01
PMCR- 2009/12/01
CRDT- 2009/12/31 06:00
PHST- 2009/08/28 00:00 [received]
PHST- 2009/10/21 00:00 [accepted]
PHST- 2009/12/31 06:00 [entrez]
PHST- 2009/12/31 06:00 [pubmed]
PHST- 2009/12/31 06:01 [medline]
PHST- 2009/12/01 00:00 [pmc-release]
AID - 10.3802/jgo.2009.20.4.243 [doi]
PST - ppublish
SO  - J Gynecol Oncol. 2009 Dec;20(4):243-5. doi: 10.3802/jgo.2009.20.4.243. Epub 2009 
      Dec 28.