PMID- 20042461
OWN - NLM
STAT- MEDLINE
DCOM- 20100319
LR  - 20191210
IS  - 1522-1466 (Electronic)
IS  - 1522-1466 (Linking)
VI  - 298
IP  - 3
DP  - 2010 Mar
TI  - IL-17A and IL-17F stimulate chemokines via MAPK pathways (ERK1/2 and p38 but not 
      JNK) in mouse cultured mesangial cells: synergy with TNF-alpha and IL-1beta.
PG  - F779-87
LID - 10.1152/ajprenal.00198.2009 [doi]
AB  - We investigated the role of IL-17 family members IL-17A and IL-17F in the 
      induction of chemokines in mouse cultured mesangial cells (SV40 MES 13 cells). We 
      evaluated the expression of the chemokines monocyte chemoattractant protein-1 
      (MCP-1) and macrophage inflammatory protein-2 (MIP-2) by ELISA and real-time 
      RT-PCR (Q-PCR). Activation of MAPK was assessed by immunoblotting. IL-17RA and 
      IL-17RC were inhibited by small interfering RNA (siRNA). We found that IL-17A or 
      IL-17F stimulation of mesangial cells led to both a dose- and time-dependent 
      increase in MCP-1 and MIP-2 release. This effect was dependent on mRNA 
      transcription and protein translation. Both also enhanced TNF-alpha- and 
      IL-1beta-mediated MCP-1 and MIP-2 release in the cells. Additionally, we observed 
      that IL-17A and IL-17F induced MAPK (p38 MAPK, ERK1/2, and JNK) activation and 
      that pharmacological inhibitors of p38 MAPK (SB203580) and ERK1/2 (U0126), but 
      not JNK (SP600125), blocked the IL-17A/IL-17F-mediated MCP-1 and MIP-2 release. 
      Mesangial cells expressed IL-17RA and IL-17RC, and the IL-17A-mediated MCP-1 and 
      MIP-2 release was significantly blocked by soluble IL-17RA. Furthermore, 
      inhibition of either IL-17RA or IL-17RC expression via siRNA led to significant 
      reduction of IL-17A/IL-17F-stimulated chemokine production. We conclude that 
      IL-17A and IL-17F induce the production of chemokines MCP-1 and MIP-2 via MAPK 
      pathways (p38 MAPK and ERK1/2), as well as mRNA transcription and protein 
      translation and have synergistic effects with TNF-alpha and IL-1beta in cultured 
      mesangial cells.
FAU - Iyoda, Masayuki
AU  - Iyoda M
AD  - Division of Nephrology, Department of Medicine, Showa University School of 
      Medicine, Tokyo, Japan. iyoda@med.showa-u.ac.jp
FAU - Shibata, Takanori
AU  - Shibata T
FAU - Kawaguchi, Mio
AU  - Kawaguchi M
FAU - Hizawa, Nobuyuki
AU  - Hizawa N
FAU - Yamaoka, Toshimitsu
AU  - Yamaoka T
FAU - Kokubu, Fumio
AU  - Kokubu F
FAU - Akizawa, Tadao
AU  - Akizawa T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091230
PL  - United States
TA  - Am J Physiol Renal Physiol
JT  - American journal of physiology. Renal physiology
JID - 100901990
RN  - 0 (Ccl2 protein, mouse)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CXCL2)
RN  - 0 (Chemokines)
RN  - 0 (Cxcl2 protein, mouse)
RN  - 0 (Il17a protein, mouse)
RN  - 0 (Il17f protein, mouse)
RN  - 0 (Il17ra protein, mouse)
RN  - 0 (Il17rc protein, mouse)
RN  - 0 (Interleukin-17)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Protein Kinase Inhibitors)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Interleukin)
RN  - 0 (Receptors, Interleukin-17)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 1)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 3)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Cell Line
MH  - Chemokine CCL2/metabolism
MH  - Chemokine CXCL2/metabolism
MH  - Chemokines/genetics/*metabolism
MH  - Enzyme Activation
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Humans
MH  - Interleukin-17/*metabolism
MH  - Interleukin-1beta/*metabolism
MH  - JNK Mitogen-Activated Protein Kinases/metabolism
MH  - Mesangial Cells/drug effects/*enzymology/*immunology
MH  - Mice
MH  - Mitogen-Activated Protein Kinase 1/metabolism
MH  - Mitogen-Activated Protein Kinase 3/metabolism
MH  - Mitogen-Activated Protein Kinases/antagonists & inhibitors/*metabolism
MH  - Polymerase Chain Reaction
MH  - Protein Kinase Inhibitors/pharmacology
MH  - RNA Interference
MH  - RNA, Messenger/metabolism
MH  - Receptors, Interleukin/genetics/metabolism
MH  - Receptors, Interleukin-17/genetics/metabolism
MH  - Recombinant Proteins/metabolism
MH  - Time Factors
MH  - Transcription, Genetic
MH  - Tumor Necrosis Factor-alpha/*metabolism
MH  - Up-Regulation
MH  - p38 Mitogen-Activated Protein Kinases/metabolism
EDAT- 2010/01/01 06:00
MHDA- 2010/03/20 06:00
CRDT- 2010/01/01 06:00
PHST- 2010/01/01 06:00 [entrez]
PHST- 2010/01/01 06:00 [pubmed]
PHST- 2010/03/20 06:00 [medline]
AID - 00198.2009 [pii]
AID - 10.1152/ajprenal.00198.2009 [doi]
PST - ppublish
SO  - Am J Physiol Renal Physiol. 2010 Mar;298(3):F779-87. doi: 
      10.1152/ajprenal.00198.2009. Epub 2009 Dec 30.