PMID- 20042584
OWN - NLM
STAT- MEDLINE
DCOM- 20100312
LR  - 20100121
IS  - 1550-6606 (Electronic)
IS  - 0022-1767 (Linking)
VI  - 184
IP  - 3
DP  - 2010 Feb 1
TI  - T cell-dendritic cell interaction dynamics during the induction of respiratory 
      tolerance and immunity.
PG  - 1317-27
LID - 10.4049/jimmunol.0902277 [doi]
AB  - Dendritic cells (DCs) residing in the lung are known to acquire inhaled Ag and, 
      after migration to the draining bronchial lymph node (brLN), to present it to 
      naive T cells in an either tolerogenic or immunogenic context. To visualize 
      endogenous lung-derived DCs, we applied fluorescent latex beads (LXs) 
      intratracheally, thereby in vivo labeling the majority of phagocytic cells within 
      the lung. Of note, LX-bearing cells subsequently arriving in the draining brLN 
      were found to represent lung-derived migratory DCs. Imaging explanted brLN by 
      two-photon laser-scanning microscopy, we quantitatively analyzed the migration 
      and interaction behavior of naive CD4(+) T cells and endogenous, lung-derived DC 
      presenting airway-delivered Ag under inflammatory or noninflammatory conditions. 
      Ag-specific naive CD4(+) T cells engaged in stable as well as transient contacts 
      with LX-bearing DCs in both situations and displayed similar overall motility 
      kinetics, including a pronounced decrease in motility at 16-20 h after antigenic 
      challenge. In contrast, the comparative analysis of T cell-DC cluster sizes as 
      well as contact durations strongly suggests that lung-derived migratory DCs and 
      naive CD4(+) T cells form more stable, long-lasting contacts under inflammatory 
      conditions favoring the induction of respiratory immunity.
FAU - Bakocevic, Nadja
AU  - Bakocevic N
AD  - Institute of Immunology, Hannover Medical School, Hannover, Germany.
FAU - Worbs, Tim
AU  - Worbs T
FAU - Davalos-Misslitz, Ana
AU  - Davalos-Misslitz A
FAU - Forster, Reinhold
AU  - Forster R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091230
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 9006-59-1 (Ovalbumin)
SB  - IM
MH  - Animals
MH  - Bronchi/immunology/pathology
MH  - CD4-Positive T-Lymphocytes/*immunology/*metabolism/pathology
MH  - Cell Communication/genetics/*immunology
MH  - Cell Migration Inhibition/genetics/immunology
MH  - Dendritic Cells/*immunology/*metabolism/pathology
MH  - Disease Models, Animal
MH  - *Immune Tolerance/genetics
MH  - *Immunity, Innate/genetics
MH  - Inflammation/genetics/immunology/pathology
MH  - Lung/cytology/*immunology/pathology
MH  - Lymph Nodes/immunology/pathology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Knockout
MH  - Mice, Transgenic
MH  - Ovalbumin/administration & dosage/immunology
EDAT- 2010/01/01 06:00
MHDA- 2010/03/13 06:00
CRDT- 2010/01/01 06:00
PHST- 2010/01/01 06:00 [entrez]
PHST- 2010/01/01 06:00 [pubmed]
PHST- 2010/03/13 06:00 [medline]
AID - jimmunol.0902277 [pii]
AID - 10.4049/jimmunol.0902277 [doi]
PST - ppublish
SO  - J Immunol. 2010 Feb 1;184(3):1317-27. doi: 10.4049/jimmunol.0902277. Epub 2009 
      Dec 30.