PMID- 20043524
OWN - NLM
STAT- MEDLINE
DCOM- 20100218
LR  - 20100101
IS  - 0043-3144 (Print)
IS  - 0043-3144 (Linking)
VI  - 58
IP  - 3
DP  - 2009 Jun
TI  - High genetic diversity in human immunodeficiency virus-type 1 in Jamaica.
PG  - 195-200
AB  - The subtypes of the human immunodeficiency virus - type 1 (HIV-1) strains from 54 
      HIV-1 - infected persons including 44 strains which were typed previously by 
      heteroduplex mobility assay (HMA) were determined by DNA sequencing and 
      phylogenetic analysis. Of 54 HIV- infected persons, 92.5% were infected with 
      HIV-1 subtype B and 7.5% with other HIV-1 subtypes including subtypes D (3.7%), A 
      (1.9%) and J (1.9%). In the phylogenetic analysis, the subtype A virus found in 
      the sample clustered with subtype A reference strains and a circulating 
      recombinant form (CRF) reference strain which originates in Central Africa and is 
      circulating in Cuba indicating a close relationship between these viruses. There 
      was 86% concordance between HMA and DNA sequencing in assigning subtype B 
      viruses. For the non-B subtype viruses, there was less concordance between the 
      two methods (67%). The results confirm the predominance of HIV-1 subtype B 
      strains and the high genetic diversity of HIV-1 strains in circulation in 
      Jamaica. The efficacies and some limitations of the HMA as a method of HIV-1 
      subtyping also were noted. It is important that the HIV/AIDS epidemic in Jamaica 
      be monitored meticulously for possible expansions in non-B subtypes and the 
      emergence of inter-subtype recombinant forms. We recommend that the more 
      expensive DNA sequencing and phylogenetic analysis, including HIV-1 genotyping 
      for antiretroviral drug resistance testing, be used as an adjunct to the more 
      cost-effective HMA to track the HIV/AIDS epidemic in Jamaica.
FAU - Heslop, O D
AU  - Heslop OD
AD  - Department of Microbiology, The University of the West Indies, Kingston 7, 
      Jamaica, West Indies. glenheslop@yahoo.com
FAU - Smikle, M F
AU  - Smikle MF
FAU - Vickers, I E
AU  - Vickers IE
FAU - Christian, N A
AU  - Christian NA
FAU - Harvey, K M
AU  - Harvey KM
FAU - Figueroa, J P
AU  - Figueroa JP
FAU - Brown, S E
AU  - Brown SE
FAU - Christie, C D C
AU  - Christie CD
FAU - Bain, B
AU  - Bain B
FAU - Barton, E N
AU  - Barton EN
LA  - eng
PT  - Journal Article
PL  - Jamaica
TA  - West Indian Med J
JT  - The West Indian medical journal
JID - 0417410
RN  - 0 (DNA, Viral)
RN  - 0 (env Gene Products, Human Immunodeficiency Virus)
RN  - 0 (gag Gene Products, Human Immunodeficiency Virus)
SB  - IM
MH  - DNA, Viral/*chemistry
MH  - *Genetic Variation
MH  - HIV Infections/*virology
MH  - HIV-1/classification/*genetics
MH  - Heteroduplex Analysis
MH  - Humans
MH  - Jamaica
MH  - Phylogeny
MH  - Reproducibility of Results
MH  - Sequence Analysis, DNA
MH  - env Gene Products, Human Immunodeficiency Virus/genetics
MH  - gag Gene Products, Human Immunodeficiency Virus/genetics
EDAT- 2010/01/02 06:00
MHDA- 2010/02/19 06:00
CRDT- 2010/01/02 06:00
PHST- 2010/01/02 06:00 [entrez]
PHST- 2010/01/02 06:00 [pubmed]
PHST- 2010/02/19 06:00 [medline]
AID - 1041 [pii]
PST - ppublish
SO  - West Indian Med J. 2009 Jun;58(3):195-200.