PMID- 20043890
OWN - NLM
STAT- MEDLINE
DCOM- 20100519
LR  - 20100215
IS  - 1872-6240 (Electronic)
IS  - 0006-8993 (Linking)
VI  - 1317
DP  - 2010 Mar 4
TI  - A long-term observation of olfactory ensheathing cells transplantation to repair 
      white matter and functional recovery in a focal ischemia model in rat.
PG  - 257-67
LID - 10.1016/j.brainres.2009.12.061 [doi]
AB  - Trials of neuroprotection in ischemic stroke mainly concern gray matter 
      protection, with little information on white matter damage. Olfactory ensheathing 
      cells (OECs) are capable of providing continuous neurotrophic factor and of 
      promoting growth and remyelination of damaged axons. We evaluated OECs for the 
      repair of white matter after transplantation in middle cerebral artery occlusion 
      (MCAO) rat. OECs were cultured from the olfactory bulbs of adult green 
      fluorescent protein (GFP)-expressing transgenic rats and transplanted into 
      peri-infarct basal ganglia imminently after reperfusion. The mortality, 
      neurological score, and function were record everyday until 56 days. At 24 h and 
      56 days, the infarction volume, the Luxol-fast blue (LFB) staining, and 
      Neurofilament (NF) immunohistochemistry and Western blot were performed to assess 
      the demyelination and remyelination in white matter. OECs transplantation reduced 
      the infarct volume, decreased mortality, and improved neurological deficits in 56 
      days after MCAO. LFB marked myelin, NF-positive immunohistochemistry, and Western 
      blot indicated that the remyelination and axon regeneration in OEC transplanted 
      rat were significant. In conclusion, OECs can protect the white matter from 
      ischemic injury, but the potential mechanisms of transplanted OEC-mediated 
      recovery need further studies to identify.
CI  - 2009 Elsevier B.V. All rights reserved.
FAU - Shi, Xianzhong
AU  - Shi X
AD  - Department of Anatomy and Embryology, Peking University Health Science Center, 
      Beijing, China 100191.
FAU - Kang, Yali
AU  - Kang Y
FAU - Hu, Qin
AU  - Hu Q
FAU - Chen, Chunhua
AU  - Chen C
FAU - Yang, Lei
AU  - Yang L
FAU - Wang, Ke
AU  - Wang K
FAU - Chen, Lin
AU  - Chen L
FAU - Huang, Hongyun
AU  - Huang H
FAU - Zhou, Changman
AU  - Zhou C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100104
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Animals
MH  - Axons/pathology/physiology
MH  - Basal Ganglia/pathology/physiopathology/surgery
MH  - Brain Ischemia/pathology/physiopathology/surgery
MH  - Demyelinating Diseases/pathology/physiopathology/surgery
MH  - Disease Models, Animal
MH  - Green Fluorescent Proteins/genetics/metabolism
MH  - Infarction, Middle Cerebral Artery/pathology/*physiopathology/*surgery
MH  - Male
MH  - Myelin Sheath/pathology/physiology
MH  - Nerve Fibers, Myelinated/pathology/*physiology
MH  - Nerve Regeneration/physiology
MH  - Neuroglia/physiology/*transplantation
MH  - Olfactory Bulb/physiology/*transplantation
MH  - Random Allocation
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Rats, Transgenic
MH  - Recovery of Function/*physiology
MH  - Time Factors
MH  - Treatment Outcome
EDAT- 2010/01/02 06:00
MHDA- 2010/05/21 06:00
CRDT- 2010/01/02 06:00
PHST- 2009/10/02 00:00 [received]
PHST- 2009/12/18 00:00 [revised]
PHST- 2009/12/19 00:00 [accepted]
PHST- 2010/01/02 06:00 [entrez]
PHST- 2010/01/02 06:00 [pubmed]
PHST- 2010/05/21 06:00 [medline]
AID - S0006-8993(09)02759-0 [pii]
AID - 10.1016/j.brainres.2009.12.061 [doi]
PST - ppublish
SO  - Brain Res. 2010 Mar 4;1317:257-67. doi: 10.1016/j.brainres.2009.12.061. Epub 2010 
      Jan 4.