PMID- 20044022
OWN - NLM
STAT- MEDLINE
DCOM- 20100617
LR  - 20100302
IS  - 1879-1107 (Electronic)
IS  - 1096-4959 (Linking)
VI  - 155
IP  - 4
DP  - 2010 Apr
TI  - Effect of plant-based feed ingredients on osmoregulation in the Atlantic salmon 
      lens.
PG  - 354-62
LID - 10.1016/j.cbpb.2009.12.002 [doi]
AB  - Lenses of adult Atlantic salmon fed with a plant oil and plant protein-based diet 
      (plant diet) were compared to lenses of fish fed a diet based on traditional 
      marine ingredients (marine diet) with respect to biochemical composition and 
      functionality ex vivo. After 12 months of feeding, plant diet-fed fish had 
      smaller lenses with higher water contents and lower concentrations of histidine 
      (His) and N-acetylhistidine (NAH) than fish fed with the marine diet. Cataract 
      development in both dietary groups was minimal and no differences between the 
      groups were observed. Lens fatty acid and lipid class composition differed 
      minimally, although a significant increase in linoleic acid was observed. The 
      lenses were examined for their ability to withstand osmotic disturbances ex vivo. 
      Culture in hypoosmotic and hyperosmotic media led to increase and decrease of 
      lens volume, respectively. Lenses from plant diet-fed fish were less resistant to 
      swelling and shrinking, released less NAH into the culture medium, and 
      accumulated His and NAH at higher rates than lenses from marine diet-fed fish. 
      Culture in hypoosmotic medium resulted in higher cataract scores than in control 
      and hyperosmotic medium. mRNA expression of selected genes, including glutathione 
      peroxidase 4 and SPARC (secreted protein acidic and rich in cysteine), was 
      affected by diet and osmotic treatment. It can be concluded that lenses of farmed 
      Atlantic salmon are affected by the diet composition, both in biochemical 
      composition and physiological functionality in relation to osmoregulation.
CI  - 2009 Elsevier Inc. All rights reserved.
FAU - Trosse, Christiane
AU  - Trosse C
AD  - National Institute of Nutrition and Seafood Research, Nordnes, Bergen, Norway. 
      ctr@nifes.no
FAU - Rhodes, Jeremy D
AU  - Rhodes JD
FAU - Sanderson, Julie
AU  - Sanderson J
FAU - Breck, Olav
AU  - Breck O
FAU - Waagbo, Rune
AU  - Waagbo R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091231
PL  - England
TA  - Comp Biochem Physiol B Biochem Mol Biol
JT  - Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology
JID - 9516061
RN  - 0 (Dietary Fats)
SB  - IM
MH  - *Animal Feed
MH  - Animals
MH  - Dietary Fats/*pharmacology
MH  - Lens, Crystalline/*chemistry/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Salmo salar/*metabolism
MH  - Water-Electrolyte Balance/*physiology
EDAT- 2010/01/02 06:00
MHDA- 2010/06/18 06:00
CRDT- 2010/01/02 06:00
PHST- 2009/08/20 00:00 [received]
PHST- 2009/12/16 00:00 [revised]
PHST- 2009/12/20 00:00 [accepted]
PHST- 2010/01/02 06:00 [entrez]
PHST- 2010/01/02 06:00 [pubmed]
PHST- 2010/06/18 06:00 [medline]
AID - S1096-4959(09)00317-0 [pii]
AID - 10.1016/j.cbpb.2009.12.002 [doi]
PST - ppublish
SO  - Comp Biochem Physiol B Biochem Mol Biol. 2010 Apr;155(4):354-62. doi: 
      10.1016/j.cbpb.2009.12.002. Epub 2009 Dec 31.