PMID- 20044941
OWN - NLM
STAT- MEDLINE
DCOM- 20100617
LR  - 20211020
IS  - 1756-9966 (Electronic)
IS  - 0392-9078 (Print)
IS  - 0392-9078 (Linking)
VI  - 29
IP  - 1
DP  - 2010 Jan 4
TI  - Evaluation of biodistribution and safety of adenovirus vector containing MDR1 in 
      mice.
PG  - 1
LID - 10.1186/1756-9966-29-1 [doi]
AB  - BACKGROUND: The aim of this study is to examine the safety and distribution of 
      Ad-EGFP-MDR1, an adenovirus encoding human multidurg resistance gene (human 
      MDR1), in the mice colon carcinoma model. METHODS: After bone marrow cells (BMCs) 
      were infected with Ad-EGFP-MDR1, they were administered by intra bone marrow-bone 
      marrow transplantation (IBM-BMT). Total adenovirus antibody and serum adenovirus 
      neutralizing factor (SNF) were determined. Biodistribution of Ad-EGFP-MDR1 was 
      detected by in situ hybridization and immunohistochemistry. The peripheral 
      hematocyte white blood cell (WBC), haemoglobin (Hb), red blood cell (RBC) and 
      platelet (Plt) counts were analyzed. RESULTS: Neither total adenovirus antibody 
      nor SNF increased weeks after BMT. In situ hybridization and immunohistochemistry 
      demonstrated concordant expression of human MDR1 and P-gp which were found in 
      lung, intestine, kidney and BMCs after BMT, but not detected in liver, spleen, 
      brain and tumor. No significant abnormality of the recovery hematocyte was 
      observed on Day 30 after treatment. CONCLUSION: The results indicate that IBM-BMT 
      administration of a replication defective adenovirus is a feasible mode of 
      delivery, allowing exogenous transference. The findings in this study are 
      conducted for the future long-term studies of safety assessment of Ad-EGFP-MDR1.
FAU - Zhao, ZhenZhen
AU  - Zhao Z
AD  - Surgery and Oncology Laboratory, Pediatric Research Institution, Children's 
      Hospital of ChongQing Medical University, ChongQing, China.
FAU - Liu, Wei
AU  - Liu W
FAU - Su, Yuxi
AU  - Su Y
FAU - Zhu, Jin
AU  - Zhu J
FAU - Zheng, GaiHuan
AU  - Zheng G
FAU - Luo, Qing
AU  - Luo Q
FAU - Jin, XianQing
AU  - Jin X
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100104
PL  - England
TA  - J Exp Clin Cancer Res
JT  - Journal of experimental & clinical cancer research : CR
JID - 8308647
RN  - 0 (ABCB1 protein, human)
RN  - 0 (ATP Binding Cassette Transporter, Subfamily B)
RN  - 0 (ATP Binding Cassette Transporter, Subfamily B, Member 1)
RN  - 0 (Antibodies, Viral)
SB  - IM
MH  - ATP Binding Cassette Transporter, Subfamily B
MH  - ATP Binding Cassette Transporter, Subfamily B, Member 1/*genetics
MH  - Adenoviridae/*genetics
MH  - Animals
MH  - Antibodies, Viral/analysis
MH  - Bone Marrow Transplantation/methods
MH  - Cell Line, Tumor
MH  - Defective Viruses/genetics
MH  - Female
MH  - Gene Transfer Techniques
MH  - *Genetic Vectors/administration & dosage/adverse effects
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Transfection
PMC - PMC2819043
EDAT- 2010/01/05 06:00
MHDA- 2010/06/18 06:00
PMCR- 2010/01/04
CRDT- 2010/01/05 06:00
PHST- 2009/08/29 00:00 [received]
PHST- 2010/01/04 00:00 [accepted]
PHST- 2010/01/05 06:00 [entrez]
PHST- 2010/01/05 06:00 [pubmed]
PHST- 2010/06/18 06:00 [medline]
PHST- 2010/01/04 00:00 [pmc-release]
AID - 1756-9966-29-1 [pii]
AID - 10.1186/1756-9966-29-1 [doi]
PST - epublish
SO  - J Exp Clin Cancer Res. 2010 Jan 4;29(1):1. doi: 10.1186/1756-9966-29-1.