PMID- 20045944
OWN - NLM
STAT- MEDLINE
DCOM- 20100608
LR  - 20231213
IS  - 1347-5215 (Electronic)
IS  - 0918-6158 (Linking)
VI  - 33
IP  - 1
DP  - 2010
TI  - Facilitated skin permeation of oregonin by elastic liposomal formulations and 
      suppression of atopic dermatitis in NC/Nga mice.
PG  - 100-6
AB  - To develop an external preparation of oregonin (ORG) for the treatment of atopic 
      dermatitis (AD), conventional creams (CC) and elastic liposomes (EL) containing 
      ORG have been formulated and examined for their in vitro skin permeation 
      properties and in vivo therapeutic efficacy assessments. EL, consisting of 
      soybean phosphatidylcholine and Tween 80 (85 : 15 w/w %), were of flexible 
      nanocarriers: they were about 130 nm in size and had a 4-fold greater 
      deformability index than conventional liposomes. In a skin permeation study using 
      a Franz diffusion cell mounted with depilated mouse skin, liposomal systems were 
      superior to cream, revealing greater flux values. Both CC and EL were diversified 
      with the addition of Trans-activating transcriptional activator (Tat) peptide, a 
      sort of cell penetrating peptide, and subjected to in vivo efficacy evaluations 
      in NC/Nga mice with AD-like lesions. On clinical observation for skin severity, 
      rapid and profound improvement was observed in the treatment group with Tat-added 
      liposomes (EL/T), showing a significant difference (p<0.05) versus Tat-added 
      cream. The results indicated that EL/T treatment is effective for normalizing the 
      immune-related responses and alleviating AD, evaluated as changes in the levels 
      of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), interleukin 
      (IL)-4, immunoglobulin E (IgE), and eosinophils in skin or blood.
FAU - Kang, Myung Joo
AU  - Kang MJ
AD  - Division of Pharmaceutical Sciences, College of Pharmacy, Chung-Ang University, 
      Seoul, Korea.
FAU - Eum, Jae Yoon
AU  - Eum JY
FAU - Jeong, Mi Sook
AU  - Jeong MS
FAU - Choi, Sun Eun
AU  - Choi SE
FAU - Park, Sang Han
AU  - Park SH
FAU - Cho, Hye In
AU  - Cho HI
FAU - Cho, Chin Sung
AU  - Cho CS
FAU - Seo, Seong Jun
AU  - Seo SJ
FAU - Lee, Min Won
AU  - Lee MW
FAU - Choi, Young Wook
AU  - Choi YW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - Biol Pharm Bull
JT  - Biological & pharmaceutical bulletin
JID - 9311984
RN  - 0 (Dermatologic Agents)
RN  - 0 (Diarylheptanoids)
RN  - 0 (Dosage Forms)
RN  - 0 (Liposomes)
RN  - 0 (Phosphatidylcholines)
RN  - 0 (Plant Extracts)
RN  - 0 (Polysorbates)
RN  - 0 (Trans-Activators)
RN  - 0 (oregonin)
SB  - IM
MH  - Administration, Topical
MH  - Alnus/*chemistry
MH  - Animals
MH  - Dermatitis, Atopic/*drug therapy/metabolism/pathology
MH  - Dermatologic Agents/administration & dosage/*pharmacology
MH  - Diarylheptanoids/pharmacology/*therapeutic use
MH  - Dosage Forms
MH  - Elasticity
MH  - Female
MH  - Immune System/drug effects
MH  - *Liposomes
MH  - Male
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Nanostructures
MH  - Permeability/drug effects
MH  - Phosphatidylcholines
MH  - Phytotherapy
MH  - Plant Extracts/pharmacology/*therapeutic use
MH  - Polysorbates
MH  - Severity of Illness Index
MH  - Skin/*drug effects
MH  - Glycine max/chemistry
MH  - Trans-Activators/pharmacology
EDAT- 2010/01/05 06:00
MHDA- 2010/06/09 06:00
CRDT- 2010/01/05 06:00
PHST- 2010/01/05 06:00 [entrez]
PHST- 2010/01/05 06:00 [pubmed]
PHST- 2010/06/09 06:00 [medline]
AID - JST.JSTAGE/bpb/33.100 [pii]
AID - 10.1248/bpb.33.100 [doi]
PST - ppublish
SO  - Biol Pharm Bull. 2010;33(1):100-6. doi: 10.1248/bpb.33.100.