PMID- 20045953
OWN - NLM
STAT- MEDLINE
DCOM- 20100608
LR  - 20190720
IS  - 1347-5215 (Electronic)
IS  - 0918-6158 (Linking)
VI  - 33
IP  - 1
DP  - 2010
TI  - The inhibitory effect of ergosterol, a bioactive constituent of a traditional 
      Japanese herbal medicine saireito on the activity of mucosal-type mast cells.
PG  - 142-5
AB  - Mucosal inflammation in ulcerative colitis (UC) is presumed to be regulated 
      primarily by type 2 T helper cell immune responses and mucosal mast cells in the 
      colon are thought to play an important role in the pathogenesis of the mucosal 
      inflammation. Saireito, a Japanese herbal medicine of standardized quality, 
      originating from traditional Chinese medicine (Kampo medicine), is composed of 
      two different Kampo medicines (shosaikoto and goreisan) and is often used for UC 
      in Japan. In this study, we examined the direct effects of these Kampo medicines 
      and their constituents on the antigen-induced degranulation of mucosal-type mast 
      cells. Mucosal-type murine bone marrow-derived mast cells (mBMMCs) were 
      pretreated by these drugs for 24 h, and immunoglobulin E (IgE) receptor-triggered 
      degranulation of mBMMCs was assessed by beta-hexosaminidase release. Goreisan 
      showed inhibitory effects on degranulation of mBMMCs in a dose-dependent manner. 
      Among the five constituent medicinal herbs of goreisan, Poria and Polyporus had 
      the inhibitory effects on mBMMCs. Ergosterol, a principal and common component of 
      Poria and Polyporus, also suppressed the degranulation of mBMMCs. Our results 
      provide a molecular basis to explain a portion of the beneficial therapeutic 
      properties of saireito on UC.
FAU - Kageyama-Yahara, Natsuko
AU  - Kageyama-Yahara N
AD  - Division of Gastrointestinal Pathophysiology, Department of Bioscience, Institute 
      of Natural Medicine, University of Toyama, Toyama, Japan.
FAU - Wang, Ping
AU  - Wang P
FAU - Wang, Xijun
AU  - Wang X
FAU - Yamamoto, Takeshi
AU  - Yamamoto T
FAU - Kadowaki, Makoto
AU  - Kadowaki M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - Biol Pharm Bull
JT  - Biological & pharmaceutical bulletin
JID - 9311984
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Receptors, IgE)
RN  - EC 3.2.1.52 (beta-N-Acetylhexosaminidases)
RN  - Z30RAY509F (Ergosterol)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents/isolation & purification/pharmacology/*therapeutic use
MH  - Cell Line
MH  - Colitis/*drug therapy/immunology/metabolism
MH  - Colon/drug effects/metabolism
MH  - Dose-Response Relationship, Drug
MH  - Drugs, Chinese Herbal/isolation & purification/pharmacology/*therapeutic use
MH  - Ergosterol/isolation & purification/pharmacology/*therapeutic use
MH  - Inflammation/*drug therapy/metabolism
MH  - Intestinal Mucosa/*drug effects/metabolism
MH  - Mast Cells/*drug effects/metabolism
MH  - Medicine, Kampo
MH  - Mice
MH  - Models, Animal
MH  - Phytotherapy
MH  - Polyporus/chemistry
MH  - Poria/chemistry
MH  - Receptors, IgE/metabolism
MH  - Th2 Cells
MH  - beta-N-Acetylhexosaminidases/metabolism
EDAT- 2010/01/05 06:00
MHDA- 2010/06/09 06:00
CRDT- 2010/01/05 06:00
PHST- 2010/01/05 06:00 [entrez]
PHST- 2010/01/05 06:00 [pubmed]
PHST- 2010/06/09 06:00 [medline]
AID - JST.JSTAGE/bpb/33.142 [pii]
AID - 10.1248/bpb.33.142 [doi]
PST - ppublish
SO  - Biol Pharm Bull. 2010;33(1):142-5. doi: 10.1248/bpb.33.142.