PMID- 2004641
OWN - NLM
STAT- MEDLINE
DCOM- 19910422
LR  - 20190707
IS  - 0014-4827 (Print)
IS  - 0014-4827 (Linking)
VI  - 193
IP  - 2
DP  - 1991 Apr
TI  - Putrescine uptake and release by a normal rat small intestine crypt cell line, 
      IEC-6.
PG  - 241-52
AB  - IEC-6 cells were cultured on permeable filter inserts with separate access to the 
      apical and basolateral sides. [3H]Putrescine uptake favored the apical side and 
      its release (in Earle's balanced salt solution containing 0.1% bovine serum 
      albumin) was six times greater in the apical-to-basolateral than in the 
      basolateral-to-apical direction. Release in DMEM did not show this preference. 
      The uptake of [3H]putrescine was stimulated approximately 1.3 times the basal 
      level by 10 mM asparagine (ASN) or 5% dialyzed fetal bovine serum whether the 
      [3H]putrescine was added at a concentration of 1 or 100 nM. The increased uptake 
      was maintained for up to 6 h. When [3H]putrescine was removed after 4 h of 
      uptake, the cells continued to release it into the medium on both sides for up to 
      4 h. Stimulated cells released only 50% as much as unstimulated cells. Unlabeled 
      putrescine reduced the uptake of [3H]putrescine with an IC50 of 1.81 x 10(-6) M 
      (r = 0.9476) and 1.02 x 10(-6) M (r = 0.9967) for unstimulated and ASN-stimulated 
      cells, respectively. When the intracellular putrescine was reduced by 
      difluoromethylornithine, the uptake of [3H]-putrescine was not changed, but its 
      release was inhibited. Sodium was not required for [3H]putrescine uptake or 
      release. Although the stimulated cells attained intracellular levels of 
      [3H]putrescine which, if expressed as concentration based on cell volume, were up 
      to 500 times the original extracellular concentration, a true concentration 
      gradient could not be proven because 85% of the [3H]putrescine was probably bound 
      to polyanions as shown by butanol extraction.
FAU - McCormack, S A
AU  - McCormack SA
AD  - Department of Physiology and Biophysics, University of Tennessee, Memphis 38163.
FAU - Johnson, L R
AU  - Johnson LR
LA  - eng
GR  - DK16505/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Exp Cell Res
JT  - Experimental cell research
JID - 0373226
RN  - 0 (Butanols)
RN  - 0 (Detergents)
RN  - 3WJQ0SDW1A (Polyethylene Glycols)
RN  - KOO5CM684H (Digitonin)
RN  - PDC6A3C0OX (Glycerol)
RN  - V10TVZ52E4 (Putrescine)
RN  - ZQN1G5V6SR (Eflornithine)
SB  - IM
MH  - Animals
MH  - Biological Transport/drug effects
MH  - Butanols/chemistry
MH  - Cell Compartmentation
MH  - Detergents
MH  - Digitonin/chemistry
MH  - Eflornithine/pharmacology
MH  - Epithelium/metabolism
MH  - Glycerol/chemistry
MH  - Intestinal Mucosa/*metabolism
MH  - Intestines/cytology
MH  - Polyethylene Glycols/chemistry
MH  - Putrescine/chemistry/*metabolism
MH  - Rats
MH  - Solubility
EDAT- 1991/04/01 00:00
MHDA- 1991/04/01 00:01
CRDT- 1991/04/01 00:00
PHST- 1991/04/01 00:00 [pubmed]
PHST- 1991/04/01 00:01 [medline]
PHST- 1991/04/01 00:00 [entrez]
AID - 10.1016/0014-4827(91)90093-a [doi]
PST - ppublish
SO  - Exp Cell Res. 1991 Apr;193(2):241-52. doi: 10.1016/0014-4827(91)90093-a.