PMID- 20046633
OWN - NLM
STAT- MEDLINE
DCOM- 20100311
LR  - 20220408
IS  - 1928-9022 (Electronic)
IS  - 0830-9000 (Print)
IS  - 0830-9000 (Linking)
VI  - 73
IP  - 4
DP  - 2009 Oct
TI  - Infection dynamics and clinical manifestations following experimental inoculation 
      of gilts at 90 days of gestation with a low dose of porcine reproductive and 
      respiratory syndrome virus.
PG  - 303-7
AB  - Understanding the dynamics of porcine reproductive and respiratory syndrome virus 
      (PRRSV) vertical transmission is important to enhance the accuracy of monitoring 
      protocols for endemically infected breeding herds. The objectives of this study 
      were to determine the prevalence of PRRSV within infected litters, to quantify 
      viremia, and to identify specific attributes of infected individuals. Eight gilts 
      were intramuscularly inoculated with 10(1) TCID(50) of a mildly virulent PRRSV 
      strain (MN-30100) at 90 d of gestation. All inoculated gilts transmitted the 
      virus in utero. The proportion of PRRSV PCR-positive piglets and the level of 
      viremia in the piglets were higher at 4 d of age than at birth or at weaning. No 
      specific attributes were associated with PRRSV infection in the piglets. This is 
      the first report, that we are aware of, documenting the efficient in utero 
      transmission of an extremely low dose of a mildly virulent strain of PRRSV. The 
      results support the sampling of piglets late during lactation as a tool to 
      monitor PRRSV shedding from sow-herds.
FAU - Cano, Jean Paul
AU  - Cano JP
AD  - Swine Disease Eradication Center, University of Minnesota, College of Veterinary 
      Medicine, St. Paul, Minnesota 55108, USA.
FAU - Dee, Scott A
AU  - Dee SA
FAU - Murtaugh, Michael P
AU  - Murtaugh MP
FAU - Rovira, Albert
AU  - Rovira A
FAU - Morrison, Robert B
AU  - Morrison RB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Canada
TA  - Can J Vet Res
JT  - Canadian journal of veterinary research = Revue canadienne de recherche 
      veterinaire
JID - 8607793
RN  - 0 (Antibodies, Viral)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Antibodies, Viral/blood
MH  - Birth Weight
MH  - Enzyme-Linked Immunosorbent Assay/veterinary
MH  - Female
MH  - Infectious Disease Transmission, Vertical/*veterinary
MH  - Logistic Models
MH  - Male
MH  - Porcine Reproductive and Respiratory Syndrome/*immunology/virology
MH  - Porcine respiratory and reproductive syndrome virus/genetics/*immunology
MH  - Pregnancy
MH  - Pregnancy Complications, Infectious/immunology/*veterinary/virology
MH  - Prevalence
MH  - RNA, Viral/chemistry/genetics
MH  - Random Allocation
MH  - Reverse Transcriptase Polymerase Chain Reaction/veterinary
MH  - Swine
MH  - Viremia/immunology/veterinary/virology
PMC - PMC2757712
EDAT- 2010/01/05 06:00
MHDA- 2010/03/12 06:00
PMCR- 2010/01/01
CRDT- 2010/01/05 06:00
PHST- 2008/01/02 00:00 [received]
PHST- 2008/09/18 00:00 [accepted]
PHST- 2010/01/05 06:00 [entrez]
PHST- 2010/01/05 06:00 [pubmed]
PHST- 2010/03/12 06:00 [medline]
PHST- 2010/01/01 00:00 [pmc-release]
AID - cjvr_10_303 [pii]
PST - ppublish
SO  - Can J Vet Res. 2009 Oct;73(4):303-7.