PMID- 20047614
OWN - NLM
STAT- MEDLINE
DCOM- 20110602
LR  - 20131121
IS  - 1399-0012 (Electronic)
IS  - 0902-0063 (Linking)
VI  - 25
IP  - 1
DP  - 2011 Jan-Feb
TI  - Bone marrow cells obtained from cirrhotic rats do not improve function or reduce 
      fibrosis in a chronic liver disease model.
PG  - 54-60
LID - 10.1111/j.1399-0012.2009.01191.x [doi]
AB  - BACKGROUND: The objective of this study was to evaluate the therapeutic potential 
      of bone marrow cells (BMCs) obtained from cirrhotic donors in a model of chronic 
      liver disease. METHODS: Chronic liver injury was induced in female Wistar rats by 
      the association of an alcoholic diet with intraperitoneal injections of carbon 
      tetrachloride. BMCs obtained from cirrhotic donors or placebo were injected 
      through the portal vein. Blood analysis of alanine aminotransferase (ALT) and 
      albumin levels, ultrasound assessment including the measurement of the portal 
      vein diameter (PVD) and liver echogenicity, histologic evaluation with 
      hematoxylin and eosin and Sirius red staining, and quantification of collagen 
      deposition were performed. RESULTS: ALT and albumin blood levels showed no 
      significant differences between the experimental groups two months after 
      injection. Additionally, no significant variation in PVD and liver echogenicity 
      was found. Histological analysis also showed no significant variation in collagen 
      deposition two months after placebo or BMC injection. CONCLUSION: This study 
      suggests that, even though BMC therapy using cells from healthy donors has 
      previously shown to be effective, this is not the case when BMCs are obtained 
      from cirrhotic animals. This result has major clinical implications when 
      considering the use of autologous BMCs from patients with chronic liver diseases.
CI  - (c) 2009 John Wiley & Sons A/S.
FAU - Mannheimer, Elida Gripp
AU  - Mannheimer EG
AD  - Centro de Cirurgia Experimental e Programa de Pos-Graduacao, Departamento de 
      Cirurgia, Faculdade de Medicina, Universidade Federal do Rio de Janeiro, Brazil.
FAU - Quintanilha, Luiz Fernando
AU  - Quintanilha LF
FAU - Carvalho, Adriana Bastos
AU  - Carvalho AB
FAU - Paredes, Bruno Diaz
AU  - Paredes BD
FAU - Goncalves de Carvalho, Felipe
AU  - Goncalves de Carvalho F
FAU - Takyia, Cristina Maeda
AU  - Takyia CM
FAU - Resende, Celia Maria Coelho
AU  - Resende CM
FAU - Ferreira da Motta Rezende, Guilherme
AU  - Ferreira da Motta Rezende G
FAU - Campos de Carvalho, Antonio Carlos
AU  - Campos de Carvalho AC
FAU - Schanaider, Alberto
AU  - Schanaider A
FAU - dos Santos Goldenberg, Regina Coeli
AU  - dos Santos Goldenberg RC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Denmark
TA  - Clin Transplant
JT  - Clinical transplantation
JID - 8710240
RN  - CL2T97X0V0 (Carbon Tetrachloride)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/*cytology/metabolism
MH  - *Bone Marrow Transplantation
MH  - Carbon Tetrachloride/toxicity
MH  - *Disease Models, Animal
MH  - End Stage Liver Disease/*complications/*therapy
MH  - Female
MH  - Liver Cirrhosis/*etiology/*pathology
MH  - Liver Function Tests
MH  - Rats
MH  - Rats, Wistar
EDAT- 2010/01/06 06:00
MHDA- 2011/06/03 06:00
CRDT- 2010/01/06 06:00
PHST- 2010/01/06 06:00 [entrez]
PHST- 2010/01/06 06:00 [pubmed]
PHST- 2011/06/03 06:00 [medline]
AID - CTR1191 [pii]
AID - 10.1111/j.1399-0012.2009.01191.x [doi]
PST - ppublish
SO  - Clin Transplant. 2011 Jan-Feb;25(1):54-60. doi: 10.1111/j.1399-0012.2009.01191.x.