PMID- 20048679
OWN - NLM
STAT- MEDLINE
DCOM- 20100326
LR  - 20200930
IS  - 1944-7884 (Electronic)
IS  - 1525-4135 (Linking)
VI  - 53
IP  - 3
DP  - 2010 Mar
TI  - T-cell dysfunction in HIV-1-infected patients with impaired recovery of CD4 cells 
      despite suppression of viral replication.
PG  - 303-10
LID - 10.1097/QAI.0b013e3181ca3f7c [doi]
AB  - INTRODUCTION: CD4 T-cell recovery is impeded in some HIVinfected patients despite 
      successful combination antiretroviral therapy (cART) with suppressed HIV RNA. We 
      hypothesized that T-cell dysfunction would be increased in these patients. 
      METHODS: In the Danish HIV Cohort Study, we identified HIV-1-infected patients 
      initiating cART with a CD4 cell count <100 cells per microliter, followed by HIV 
      RNA <50 copies per milliliter for 3 years. Patients with a CD4 count <200 cells 
      per microliter after 3 years were identified as cases; 42 patients with a CD4 
      count > or = 200 cells per microliter were selected as controls. Six-color flow 
      cytometry was performed on whole blood. Cytokine levels in supernatants from 
      whole blood stimulations were assessed. RESULTS: The case and control groups 
      comprised 18 and 35 patients, respectively. Cases were older than controls 
      (median: 54/46 years). The fraction of CD28+ cells was decreased among cases in 
      the CD4+ and CD8+ T-cell subsets (P = 0.0014/P = 0.0349) and in the corresponding 
      naive subsets (P = 0.0011/P , 0.0001). Cases had higher expression of human 
      leukocyte antigen (HLA)-DR on naive CD4 and CD8 T cells (P = 0.0007/P = 0.0028). 
      The production of interleukin (IL)-10 and IL-2 to phytohemagglutinin was 
      decreased in cases (P < 0.0001/P = 0.019). CONCLUSIONS: Patients with impaired 
      CD4 recovery shared a dysregulated T-cell phenotype with low CD28, high HLA-DR 
      expression, and low IL-2 and IL-10 production.
FAU - Erikstrup, Christian
AU  - Erikstrup C
AD  - Department of Clinical Immunology, Aarhus University Hospital, Skejby, 
      Brendstrupgaardsvej 100, DK-8200 Aarhus N, Denmark. christian@erikstrup.org
FAU - Kronborg, Gitte
AU  - Kronborg G
FAU - Lohse, Nicolai
AU  - Lohse N
FAU - Ostrowski, Sisse Rye
AU  - Ostrowski SR
FAU - Gerstoft, Jan
AU  - Gerstoft J
FAU - Ullum, Henrik
AU  - Ullum H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Acquir Immune Defic Syndr
JT  - Journal of acquired immune deficiency syndromes (1999)
JID - 100892005
RN  - 0 (Anti-HIV Agents)
RN  - 0 (CD28 Antigens)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (Interleukin-2)
RN  - 130068-27-8 (Interleukin-10)
SB  - IM
MH  - Adult
MH  - Anti-HIV Agents/*therapeutic use
MH  - CD28 Antigens/analysis
MH  - CD4 Lymphocyte Count
MH  - CD4-Positive T-Lymphocytes/chemistry/*immunology
MH  - Denmark
MH  - Female
MH  - Flow Cytometry
MH  - HIV Infections/*drug therapy/*immunology
MH  - HLA-DR Antigens/analysis
MH  - Humans
MH  - Interleukin-10/metabolism
MH  - Interleukin-2/metabolism
MH  - Male
MH  - Middle Aged
MH  - *Viral Load
EDAT- 2010/01/06 06:00
MHDA- 2010/03/27 06:00
CRDT- 2010/01/06 06:00
PHST- 2010/01/06 06:00 [entrez]
PHST- 2010/01/06 06:00 [pubmed]
PHST- 2010/03/27 06:00 [medline]
AID - 10.1097/QAI.0b013e3181ca3f7c [doi]
PST - ppublish
SO  - J Acquir Immune Defic Syndr. 2010 Mar;53(3):303-10. doi: 
      10.1097/QAI.0b013e3181ca3f7c.