PMID- 20051306 OWN - NLM STAT- MEDLINE DCOM- 20100330 LR - 20161125 IS - 1879-3460 (Electronic) IS - 0168-1605 (Linking) VI - 137 IP - 2-3 DP - 2010 Feb 28 TI - Selection of autochthonous Oenococcus oeni strains according to their oenological properties and vinification results. PG - 230-5 LID - 10.1016/j.ijfoodmicro.2009.11.027 [doi] AB - The goal of this study is to carry out a characterization of 84 Oenococcus oeni strains isolated from Tempranillo wine samples taken at the cellars in Castilla-La Mancha, in order to select those showing the highest potential as oenological starter cultures. Various oenological properties were analyzed and the ability of some of these strains to grow and undergo MLF in simulated laboratory microvinifications was tested. Twenty-two strains were selected on the basis of fermentation assays and the eight that produced the best results in the chemical analysis of the wines were chosen for further assays. None of the eight strains was either able to produce biogenic amines or displayed tannase or anthocyanase activities. On the other hand all presented activity against p-NP-beta Glucopyranoside, p-NP-alpha Glucopyranoside and p-NP-beta xylopyranoside. Randomly Amplified Polymorphic DNA (RAPD)-PCR was used to determine the colonizing ability of the inoculated strains. C22L9 and D13L13 strains showed the highest implantation values. On the basis of this characterization, two strains have been selected which are suitable as starter cultures for MLF of Tempranillo wine. Use of these strains will ensure that MLF proceeds successfully and gives retention of the organoleptic characteristics of wines made in Castilla-La Mancha. CI - (c) 2009 Elsevier B.V. All rights reserved. FAU - Ruiz, Patricia AU - Ruiz P AD - Departamento de Quimica Analitica y Tecnologia de Alimentos, Facultad de Ciencias del Medio Ambiente, Universidad de Castilla-La Mancha, Avda. Carlos III s/n, 45071, Toledo, Spain. FAU - Izquierdo, Pedro Miguel AU - Izquierdo PM FAU - Sesena, Susana AU - Sesena S FAU - Palop, Maria Llanos AU - Palop ML LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20091202 PL - Netherlands TA - Int J Food Microbiol JT - International journal of food microbiology JID - 8412849 RN - 0 (Anthocyanins) RN - 0 (Biogenic Amines) RN - 0 (Malates) RN - 33X04XA5AT (Lactic Acid) RN - 817L1N4CKP (malic acid) RN - EC 3.1.1.- (Carboxylic Ester Hydrolases) RN - EC 3.1.1.20 (tannase) SB - IM MH - Anthocyanins/metabolism MH - Biogenic Amines/metabolism MH - Carboxylic Ester Hydrolases/metabolism MH - Fermentation MH - Lactic Acid/*metabolism MH - Malates/*metabolism MH - Oenococcus/*genetics/isolation & purification/*metabolism MH - Random Amplified Polymorphic DNA Technique MH - Spain MH - Wine/*microbiology EDAT- 2010/01/07 06:00 MHDA- 2010/03/31 06:00 CRDT- 2010/01/07 06:00 PHST- 2009/07/23 00:00 [received] PHST- 2009/11/13 00:00 [revised] PHST- 2009/11/26 00:00 [accepted] PHST- 2010/01/07 06:00 [entrez] PHST- 2010/01/07 06:00 [pubmed] PHST- 2010/03/31 06:00 [medline] AID - S0168-1605(09)00621-7 [pii] AID - 10.1016/j.ijfoodmicro.2009.11.027 [doi] PST - ppublish SO - Int J Food Microbiol. 2010 Feb 28;137(2-3):230-5. doi: 10.1016/j.ijfoodmicro.2009.11.027. Epub 2009 Dec 2.