PMID- 20052599
OWN - NLM
STAT- MEDLINE
DCOM- 20100326
LR  - 20220316
IS  - 1432-8798 (Electronic)
IS  - 0304-8608 (Linking)
VI  - 155
IP  - 3
DP  - 2010 Mar
TI  - GP4 of porcine reproductive and respiratory syndrome virus contains a 
      neutralizing epitope that is susceptible to immunoselection in vitro.
PG  - 371-8
LID - 10.1007/s00705-009-0582-7 [doi]
AB  - Glycoprotein 4 (GP4) of porcine reproductive and respiratory syndrome virus 
      (PRRSV) contains a highly variable neutralizing epitope. The present study aimed 
      to investigate whether this epitope is susceptible to immunoselection by 
      antibodies in vitro. Cultivation of PRRSV in vitro in the continuous presence of 
      neutralizing monoclonal antibodies (mAbs) directed against this epitope resulted 
      in the selection of mAb-resistant PRRSV strains within five passages. Comparison 
      of the GP4 amino acid (aa) sequence of the original PRRSV strain with the GP4 aa 
      sequences of the mAb-resistant PRRSV strains revealed aa substitutions within 
      this epitope. In conclusion, this study shows that the neutralizing epitope on 
      GP4 is susceptible to immunoselection by antibodies in vitro.
FAU - Costers, Sarah
AU  - Costers S
AD  - Laboratory of Virology, Faculty of Veterinary Medicine, Ghent University, 
      Salisburylaan 133, Merelbeke, Belgium. sarah.costers@ugent.be
FAU - Lefebvre, David J
AU  - Lefebvre DJ
FAU - Van Doorsselaere, Jan
AU  - Van Doorsselaere J
FAU - Vanhee, Merijn
AU  - Vanhee M
FAU - Delputte, Peter L
AU  - Delputte PL
FAU - Nauwynck, Hans J
AU  - Nauwynck HJ
LA  - eng
SI  - GENBANK/FJ004949
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100107
PL  - Austria
TA  - Arch Virol
JT  - Archives of virology
JID - 7506870
RN  - 0 (Antibodies, Neutralizing)
RN  - 0 (Antibodies, Viral)
RN  - 0 (Epitopes, B-Lymphocyte)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Amino Acid Substitution/genetics
MH  - Animals
MH  - Antibodies, Neutralizing/*immunology
MH  - Antibodies, Viral/*immunology
MH  - Cell Line
MH  - DNA Mutational Analysis
MH  - Epitopes, B-Lymphocyte/*immunology
MH  - Molecular Sequence Data
MH  - Mutation, Missense
MH  - Porcine respiratory and reproductive syndrome virus/*immunology
MH  - *Selection, Genetic
MH  - Sequence Analysis, DNA
MH  - Serial Passage
MH  - Swine
MH  - Viral Proteins/*immunology
EDAT- 2010/01/07 06:00
MHDA- 2010/03/27 06:00
CRDT- 2010/01/07 06:00
PHST- 2009/01/27 00:00 [received]
PHST- 2009/12/01 00:00 [accepted]
PHST- 2010/01/07 06:00 [entrez]
PHST- 2010/01/07 06:00 [pubmed]
PHST- 2010/03/27 06:00 [medline]
AID - 10.1007/s00705-009-0582-7 [doi]
PST - ppublish
SO  - Arch Virol. 2010 Mar;155(3):371-8. doi: 10.1007/s00705-009-0582-7. Epub 2010 Jan 
      7.