PMID- 20053461
OWN - NLM
STAT- MEDLINE
DCOM- 20100803
LR  - 20231213
IS  - 1873-2534 (Electronic)
IS  - 0165-2427 (Linking)
VI  - 135
IP  - 3-4
DP  - 2010 Jun 15
TI  - Immunogenicity of recombinant GP5 protein of porcine reproductive and respiratory 
      syndrome virus expressed in tobacco plant.
PG  - 234-42
LID - 10.1016/j.vetimm.2009.12.003 [doi]
AB  - The aim of the study was to evaluate the immunogenicity of the ORF5-encoded major 
      envelop glycoprotein 5 (GP5) of porcine reproductive and respiratory syndrome 
      virus (PRRSV) expressed in tobacco plant as a potential pig oral vaccine in 
      protection against PRRSV infection. Six-week-old PRRSV-free pigs were fed four 
      times orally with 50g of chopped fresh GP5 transgenic tobacco leaves (GP5-T) (GP5 
      reaching 0.011% of total soluble protein) or wild-type tobacco leaves (W-T) each 
      on days 0, 14, 28, and 42. Samples of serum, saliva, and peripheral blood 
      mononuclear cells (PBMCs) were collected on days -1, 6, 13, 20, 27, 34, 41, and 
      48 after the initial oral vaccination. A similar vaccination-dependent gradual 
      increase in the responses of serum and saliva anti-PRRSV total IgG and IgA, 
      respectively, and in the levels of PRRSV-specific blastogenic response of PBMCs 
      was seen in GP5-T-treated pigs; all statistically significant elevations occurred 
      after the 2nd vaccination and were revealed after 20 days post-initial oral 
      vaccination (DPIOV). Pigs fed on GP5-T also developed serum neutralizing 
      antibodies to PRRSV at a titer of 1:4-1:8 after the 4th vaccination by 48 DPIOV. 
      No detectable anti-PRRSV antibody responses and PRRSV-specific blastogenic 
      response were seen in W-T-treated pigs. The present study has demonstrated that 
      pigs fed on GP5-T could develop specific mucosal as well as systemic humoral and 
      cellular immune responses against PRRSV. The results also support that transgenic 
      plant as GP5-T can be an effective system for oral delivery of recombinant 
      subunit vaccines in pigs.
CI  - Copyright 2009 Elsevier B.V. All rights reserved.
FAU - Chia, Min-Yuan
AU  - Chia MY
AD  - Graduate Institute of Veterinary Medicine, School of Veterinary Medicine, Taipei 
      106, Taiwan, ROC.
FAU - Hsiao, Shih-Hsuan
AU  - Hsiao SH
FAU - Chan, Hui-Ting
AU  - Chan HT
FAU - Do, Yi-Yin
AU  - Do YY
FAU - Huang, Pung-Ling
AU  - Huang PL
FAU - Chang, Hui-Wen
AU  - Chang HW
FAU - Tsai, Yi-Chieh
AU  - Tsai YC
FAU - Lin, Chun-Ming
AU  - Lin CM
FAU - Pang, Victor Fei
AU  - Pang VF
FAU - Jeng, Chian-Ren
AU  - Jeng CR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091222
PL  - Netherlands
TA  - Vet Immunol Immunopathol
JT  - Veterinary immunology and immunopathology
JID - 8002006
RN  - 0 (Antibodies, Neutralizing)
RN  - 0 (Antibodies, Viral)
RN  - 0 (DNA, Viral)
RN  - 0 (Immunoglobulin A, Secretory)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Vaccines, Edible)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (Viral Vaccines)
RN  - 0 (glycoprotein 5, PRRSV)
SB  - IM
MH  - Administration, Oral
MH  - Animals
MH  - Antibodies, Neutralizing/biosynthesis/blood
MH  - Antibodies, Viral/biosynthesis/blood
MH  - Base Sequence
MH  - Bioreactors
MH  - DNA, Viral/genetics
MH  - Immunity, Cellular
MH  - Immunity, Humoral
MH  - Immunity, Mucosal
MH  - Immunoglobulin A, Secretory/biosynthesis
MH  - Immunoglobulin G/biosynthesis/blood
MH  - Lymphocyte Activation
MH  - Male
MH  - Plants, Genetically Modified
MH  - Porcine Reproductive and Respiratory Syndrome/immunology/prevention & control
MH  - Porcine respiratory and reproductive syndrome virus/*genetics/*immunology
MH  - Saliva/immunology
MH  - Sus scrofa
MH  - Swine
MH  - Nicotiana/*genetics
MH  - Vaccines, Edible/administration & dosage/genetics
MH  - Viral Envelope Proteins/*genetics/*immunology
MH  - Viral Vaccines/*administration & dosage/*genetics
EDAT- 2010/01/08 06:00
MHDA- 2010/08/04 06:00
CRDT- 2010/01/08 06:00
PHST- 2009/08/25 00:00 [received]
PHST- 2009/12/04 00:00 [revised]
PHST- 2009/12/13 00:00 [accepted]
PHST- 2010/01/08 06:00 [entrez]
PHST- 2010/01/08 06:00 [pubmed]
PHST- 2010/08/04 06:00 [medline]
AID - S0165-2427(09)00420-6 [pii]
AID - 10.1016/j.vetimm.2009.12.003 [doi]
PST - ppublish
SO  - Vet Immunol Immunopathol. 2010 Jun 15;135(3-4):234-42. doi: 
      10.1016/j.vetimm.2009.12.003. Epub 2009 Dec 22.