PMID- 20053505
OWN - NLM
STAT- MEDLINE
DCOM- 20101115
LR  - 20131121
IS  - 1873-2542 (Electronic)
IS  - 0378-1135 (Linking)
VI  - 143
IP  - 2-4
DP  - 2010 Jul 14
TI  - A molecular analysis of European porcine reproductive and respiratory syndrome 
      virus isolated in South Korea.
PG  - 394-400
LID - 10.1016/j.vetmic.2009.11.039 [doi]
AB  - In a situation where European genotype of porcine reproductive and respiratory 
      syndrome virus (PRRSV) has recently emerged in South Korea, this study aims to 
      understand variations in and relatedness among 25 European (EU) genotype 1 PRRSV 
      isolates obtained from Korean pig farms during the period ranging from 2006 to 
      2009 for their sequences of nonstructural protein 2 (NSP2), open reading frames 
      (ORF) 5 and 7, which, in turn, were compared with those of published Korean type 
      1 PRRSV isolates (CP6874, IV3140 and KNU07) and other EU PRRSV strains. The 
      sequence data revealed that all Korean type 1 isolates were found to possess 
      notable 19 amino acid deletions within NSP2 between positions 748 and 766. Based 
      on the complete ORF5 sequences, the results showed that the Korean isolates 
      amounted to 82.0-99.5% in amino acid identity with one another, while sharing a 
      lower level of amino acid identity ranging from 71.6% to 92.0% with EU genotype 
      strains isolated in other geographic areas. According to an amino acid sequence 
      comparison of ORF7, the level of identity among the Korean type 1 isolates was 
      found to range from 86.7% to 100%. Phylogenetic analyses based on ORF5 and ORF7 
      sequences indicated that the Korean type 1 isolates belonged to the pan-European 
      subtype 1; in ORF5 phylogeny, they form three distinct clusters from other EU 
      genotype PRRSV strains. In conclusion, those findings suggest that the Korean 
      type 1 PRRSV may have undergone a high degree of variations since EU genotype 
      virus was first detected.
CI  - (c) 2009 Elsevier B.V. All rights reserved.
FAU - Kim, Seong-Hee
AU  - Kim SH
AD  - Animal Disease Diagnostic Center, National Veterinary Research and Quarantine 
      Services, Juangang-ro, Anyang 430-824, Republic of Korea.
FAU - Roh, In-Soon
AU  - Roh IS
FAU - Choi, Eun-Jin
AU  - Choi EJ
FAU - Lee, Changhee
AU  - Lee C
FAU - Lee, Chang-Hee
AU  - Lee CH
FAU - Lee, Kyoung-Hyun
AU  - Lee KH
FAU - Lee, Kyoung-Ki
AU  - Lee KK
FAU - Song, Yoon-Kyung
AU  - Song YK
FAU - Lee, O-Soo
AU  - Lee OS
FAU - Park, Choi-Kyu
AU  - Park CK
LA  - eng
PT  - Journal Article
DEP - 20091204
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (Viral Nonstructural Proteins)
RN  - 0 (Viral Proteins)
RN  - 0 (glycoprotein 5, PRRSV)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Europe/epidemiology
MH  - Gene Expression Regulation, Viral/physiology
MH  - Genetic Variation
MH  - Genotype
MH  - Lung/virology
MH  - Lymph Nodes/virology
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - Porcine Reproductive and Respiratory Syndrome/epidemiology/pathology/*virology
MH  - Porcine respiratory and reproductive syndrome virus/*classification/*genetics
MH  - Republic of Korea/epidemiology
MH  - Swine
MH  - Time Factors
MH  - Viral Envelope Proteins
MH  - Viral Nonstructural Proteins/genetics
MH  - Viral Proteins/genetics/metabolism
EDAT- 2010/01/08 06:00
MHDA- 2010/11/16 06:00
CRDT- 2010/01/08 06:00
PHST- 2009/08/28 00:00 [received]
PHST- 2009/11/20 00:00 [revised]
PHST- 2009/11/26 00:00 [accepted]
PHST- 2010/01/08 06:00 [entrez]
PHST- 2010/01/08 06:00 [pubmed]
PHST- 2010/11/16 06:00 [medline]
AID - S0378-1135(09)00588-4 [pii]
AID - 10.1016/j.vetmic.2009.11.039 [doi]
PST - ppublish
SO  - Vet Microbiol. 2010 Jul 14;143(2-4):394-400. doi: 10.1016/j.vetmic.2009.11.039. 
      Epub 2009 Dec 4.