PMID- 20056132
OWN - NLM
STAT- MEDLINE
DCOM- 20100405
LR  - 20131121
IS  - 1872-7972 (Electronic)
IS  - 0304-3940 (Linking)
VI  - 471
IP  - 1
DP  - 2010 Feb 26
TI  - DNA damage after intracerebroventricular injection of ouabain in rats.
PG  - 6-9
LID - 10.1016/j.neulet.2009.12.072 [doi]
AB  - There is an emerging body of data suggesting that bipolar disorder is associated 
      with DNA damage. Intracerebroventricular (i.c.v.) administration of ouabain in 
      rats results in manic-like alterations. We evaluated DNA damage of peripheral 
      blood, cerebrospinal fluid and hippocampus of rats after i.c.v. ouabain 
      injection. Ouabain-induced hyperlocomotion was examined in an open field. 
      Additionally, we used single cell gel electrophoresis (comet assay) to measure 
      early transient damage in cerebrospinal fluid (CSF), hippocampus and blood; and 
      the micronucleus test to measure persistent damage in total blood samples of rats 
      after ouabain administration. Our findings demonstrated that ouabain induced 
      hyperlocomotion in rats, and this response remained up to 7 days following a 
      single i.c.v. injection. In addition, we observed that the persistent increase in 
      the rat spontaneous locomotion is associated with increased hippocampal and 
      peripheral index of early DNA damage in rats. No significant alterations were 
      observed in the micronucleus frequency in total blood samples of the rats after 
      the ouabain i.c.v. injection. These results suggest that ouabain may induce 
      peripheral and central early DNA damage, but this early damage may be repaired.
CI  - 2010 Elsevier Ireland Ltd. All rights reserved.
FAU - Jornada, Luciano K
AU  - Jornada LK
AD  - Laboratory of Neurosciences and National Institute for Translational Medicine, 
      University of Southern Santa Catarina, Criciuma, SC, Brazil.
FAU - Valvassori, Samira S
AU  - Valvassori SS
FAU - Arent, Camila O
AU  - Arent CO
FAU - Leffa, Daniela
AU  - Leffa D
FAU - Damiani, Adriani A
AU  - Damiani AA
FAU - Hainzenreder, Giana
AU  - Hainzenreder G
FAU - Ferreira, Camila L
AU  - Ferreira CL
FAU - Moretti, Morgana
AU  - Moretti M
FAU - Andrade, Vanessa M
AU  - Andrade VM
FAU - Quevedo, Joao
AU  - Quevedo J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100105
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - 5ACL011P69 (Ouabain)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Bipolar Disorder/chemically induced/*genetics/psychology
MH  - Comet Assay
MH  - DNA/blood/cerebrospinal fluid/metabolism
MH  - *DNA Damage
MH  - Disease Models, Animal
MH  - Hippocampus/metabolism
MH  - Injections, Intraventricular
MH  - Male
MH  - Micronucleus Tests
MH  - Motor Activity
MH  - *Ouabain
MH  - Rats
MH  - Rats, Wistar
EDAT- 2010/01/09 06:00
MHDA- 2010/04/07 06:00
CRDT- 2010/01/09 06:00
PHST- 2009/09/12 00:00 [received]
PHST- 2009/12/01 00:00 [revised]
PHST- 2009/12/29 00:00 [accepted]
PHST- 2010/01/09 06:00 [entrez]
PHST- 2010/01/09 06:00 [pubmed]
PHST- 2010/04/07 06:00 [medline]
AID - S0304-3940(09)01676-0 [pii]
AID - 10.1016/j.neulet.2009.12.072 [doi]
PST - ppublish
SO  - Neurosci Lett. 2010 Feb 26;471(1):6-9. doi: 10.1016/j.neulet.2009.12.072. Epub 
      2010 Jan 5.