PMID- 20060537
OWN - NLM
STAT- MEDLINE
DCOM- 20100413
LR  - 20201217
IS  - 1873-3778 (Electronic)
IS  - 0021-9673 (Linking)
VI  - 1217
IP  - 8
DP  - 2010 Feb 19
TI  - On-line identification of 4''-isovalerylspiramycin I in the genetic engineered 
      strain of S. spiramyceticus F21 by liquid chromatography with electrospray 
      ionization tandem mass spectrometry, ultraviolet absorbance detection and nuclear 
      magnetic resonance spectrometry.
PG  - 1419-24
LID - 10.1016/j.chroma.2009.12.072 [doi]
AB  - LC-hyphenated techniques were applied to the on-line identification of 
      isovalerylspiramycin I (isp I), a spiramycin-like macrolide in the crude extract 
      of fermentation broth from a genetically engineered strain of S. spiramyceticus 
      F21. In the structural characterization of the large molecular secondary 
      metabolite of isp I, LC-DAD-UV-ESI-MS(n) analysis played a crucial role, and 
      stop-flow LC-(1)H NMR measurement, with bitespiramycin used as reference, was a 
      valuable complement approach. This rational approach proved to be an efficient 
      means for the rapid and accurate structural determination of known microbial 
      secondary metabolites, by which targeted isolation of component(s) of interest 
      can be subsequently performed for further biological and pharmacological studies 
      in drug development.
CI  - Copyright 2009 Elsevier B.V. All rights reserved.
FAU - Li, Jingyan
AU  - Li J
AD  - Key Laboratory of Biotechnology of Antibiotics, Ministry of Health, Peking Union 
      Medical College & Chinese Academy of Medical Sciences, Beijing 100050, China.
FAU - Ma, Chunyan
AU  - Ma C
FAU - Wang, Hongyuan
AU  - Wang H
FAU - Wang, Yinghong
AU  - Wang Y
FAU - Wu, Linzhuan
AU  - Wu L
FAU - Wang, Yiguang
AU  - Wang Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Validation Study
DEP - 20100104
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 8025-81-8 (Spiramycin)
RN  - K5E0V85NB9 (carrimycin)
SB  - IM
MH  - Chromatography, Liquid/economics/*methods
MH  - Magnetic Resonance Spectroscopy/economics/*methods
MH  - Sensitivity and Specificity
MH  - Spectrometry, Mass, Electrospray Ionization/economics/*methods
MH  - Spiramycin/*analogs & derivatives/analysis
MH  - Streptomyces/*chemistry/genetics
MH  - Tandem Mass Spectrometry/economics/methods
MH  - Time Factors
EDAT- 2010/01/12 06:00
MHDA- 2010/04/14 06:00
CRDT- 2010/01/12 06:00
PHST- 2009/04/22 00:00 [received]
PHST- 2009/11/26 00:00 [revised]
PHST- 2009/12/22 00:00 [accepted]
PHST- 2010/01/12 06:00 [entrez]
PHST- 2010/01/12 06:00 [pubmed]
PHST- 2010/04/14 06:00 [medline]
AID - S0021-9673(09)01922-0 [pii]
AID - 10.1016/j.chroma.2009.12.072 [doi]
PST - ppublish
SO  - J Chromatogr A. 2010 Feb 19;1217(8):1419-24. doi: 10.1016/j.chroma.2009.12.072. 
      Epub 2010 Jan 4.