PMID- 20061554
OWN - NLM
STAT- MEDLINE
DCOM- 20100415
LR  - 20211020
IS  - 1532-298X (Electronic)
IS  - 1040-4651 (Print)
IS  - 1040-4651 (Linking)
VI  - 22
IP  - 1
DP  - 2010 Jan
TI  - Arabidopsis CULLIN4-damaged DNA binding protein 1 interacts with CONSTITUTIVELY 
      PHOTOMORPHOGENIC1-SUPPRESSOR OF PHYA complexes to regulate photomorphogenesis and 
      flowering time.
PG  - 108-23
LID - 10.1105/tpc.109.065490 [doi]
AB  - CONSTITUTIVELY PHOTOMORPHOGENIC1 (COP1) possesses E3 ligase activity and promotes 
      degradation of key factors involved in the light regulation of plant development. 
      The finding that CULLIN4 (CUL4)-Damaged DNA Binding Protein1 (DDB1) interacts 
      with DDB1 binding WD40 (DWD) proteins to act as E3 ligases implied that CUL4-DDB1 
      may associate with COP1-SUPPRESSOR OF PHYA (SPA) protein complexes, since COP1 
      and SPAs are DWD proteins. Here, we demonstrate that CUL4-DDB1 physically 
      associates with COP1-SPA complexes in vitro and in vivo, likely via direct 
      interaction of DDB1 with COP1 and SPAs. The interactions between DDB1 and COP1, 
      SPA1, and SPA3 were disrupted by mutations in the WDXR motifs of MBP-COP1, 
      His-SPA1, and His-SPA3. CUL4 cosuppression mutants enhanced weak cop1 
      photomorphogenesis and flowered early under short days. Early flowering of short 
      day-grown cul4 mutants correlated with increased FLOWERING LOCUS T transcript 
      levels, whereas CONSTANS transcript levels were not altered. De-etiolated1 and 
      COP1 can bind DDB1 and may work with CUL4-DDB1 in distinct complexes, but they 
      mediate photomorphogenesis in concert. Thus, a series of CUL4-DDB1-COP1-SPA E3 
      ligase complexes may mediate the repression of photomorphogenesis and, possibly, 
      of flowering time.
FAU - Chen, Haodong
AU  - Chen H
AD  - Peking-Yale Joint Center for Plant Molecular Genetics and Agro-biotechnology, 
      National Laboratory of Protein Engineering and Plant Genetic Engineering, College 
      of Life Sciences, Peking University, Beijing 100871, China.
FAU - Huang, Xi
AU  - Huang X
FAU - Gusmaroli, Giuliana
AU  - Gusmaroli G
FAU - Terzaghi, William
AU  - Terzaghi W
FAU - Lau, On Sun
AU  - Lau OS
FAU - Yanagawa, Yuki
AU  - Yanagawa Y
FAU - Zhang, Yu
AU  - Zhang Y
FAU - Li, Jigang
AU  - Li J
FAU - Lee, Jae-Hoon
AU  - Lee JH
FAU - Zhu, Danmeng
AU  - Zhu D
FAU - Deng, Xing Wang
AU  - Deng XW
LA  - eng
GR  - R01 GM047850/GM/NIGMS NIH HHS/United States
GR  - R37 GM047850/GM/NIGMS NIH HHS/United States
GR  - GM47850/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20100108
PL  - England
TA  - Plant Cell
JT  - The Plant cell
JID - 9208688
RN  - 0 (Arabidopsis Proteins)
RN  - 0 (CULLIN4 protein, Arabidopsis)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Cullin Proteins)
RN  - 0 (DDB1a protein, Arabidopsis)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (RNA, Plant)
RN  - 0 (SPA1 protein, Arabidopsis)
RN  - EC 2.3.2.27 (AT2G32950 protein, Arabidopsis)
RN  - EC 2.3.2.27 (Ubiquitin-Protein Ligases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Arabidopsis/*genetics/growth & development/metabolism
MH  - Arabidopsis Proteins/genetics/*metabolism
MH  - Cell Cycle Proteins/genetics/metabolism
MH  - Cullin Proteins/genetics/metabolism
MH  - DNA-Binding Proteins/genetics/*metabolism
MH  - Flowers/genetics/*growth & development/metabolism
MH  - Gene Expression Regulation, Developmental
MH  - Gene Expression Regulation, Plant
MH  - Light
MH  - Molecular Sequence Data
MH  - Mutation
MH  - Plants, Genetically Modified/genetics/growth & development/metabolism
MH  - RNA, Plant/genetics
MH  - Ubiquitin-Protein Ligases/genetics/*metabolism
PMC - PMC2828697
EDAT- 2010/01/12 06:00
MHDA- 2010/04/16 06:00
PMCR- 2011/01/01
CRDT- 2010/01/12 06:00
PHST- 2010/01/12 06:00 [entrez]
PHST- 2010/01/12 06:00 [pubmed]
PHST- 2010/04/16 06:00 [medline]
PHST- 2011/01/01 00:00 [pmc-release]
AID - tpc.109.065490 [pii]
AID - 065490 [pii]
AID - 10.1105/tpc.109.065490 [doi]
PST - ppublish
SO  - Plant Cell. 2010 Jan;22(1):108-23. doi: 10.1105/tpc.109.065490. Epub 2010 Jan 8.