PMID- 20063118
OWN - NLM
STAT- MEDLINE
DCOM- 20100421
LR  - 20211020
IS  - 1572-994X (Electronic)
IS  - 0920-8569 (Linking)
VI  - 40
IP  - 2
DP  - 2010 Apr
TI  - Prevalence and phylogenetic analysis of the isolated type I porcine reproductive 
      and respiratory syndrome virus from 2007 to 2008 in Korea.
PG  - 225-30
LID - 10.1007/s11262-009-0433-3 [doi]
AB  - The first Korean strain of porcine reproductive and respiratory syndrome virus 
      (PRRSV) was isolated in 1997, and it exhibited high similarity to strain VR-2332 
      (type II PRRSV; North American type). Recently, however, infection with type I 
      PRRSV (European type) has also been reported in Korea. To date, preliminary data 
      about type I PRRSV prevalence in Korea have not been reported. Here, using 
      reverse transcriptase (RT)-PCR, we analyzed 383 archived field samples from 101 
      pig farms in Korea that were collected from 2007 to 2008. We identified 155 
      samples from 68 farms that were positive for PRRSV. Fifty-one samples (51/155; 
      32.9%) and 20 farms (20/68; 29.4%) were type I PRRSV-positive/type II 
      PRRSV-negative. Furthermore, we tried to isolate the type I PRRSV from positive 
      samples and seven type I PRRSV were isolated using PAM. The phylogenetic analysis 
      using the type I PRRSV isolates (7 isolates) was performed based on open reading 
      frame (ORF)5 (accession numbers GU325642 to GU325648) and ORF7 (accession numbers 
      GU325635 to GU325641). In the phylogenetic study, seven type I PRRSV isolates 
      were closely related with panEuropean based on ORF7, while they were genetically 
      distinct from Lelystad virus and made a unique clade based on ORF5. The results 
      of this study demonstrate that infection with type I PRRSV is not uncommon in 
      Korean pig farms, which suggests that diagnosis and control of type I PRRSV 
      should be considered in Korea. A new approach to vaccination against, and 
      epidemiological analysis of, Korean PRRSV is urgently needed.
FAU - Lee, Chulseung
AU  - Lee C
AD  - Chung-Aang University School of Medicine, Seoul 156-756, Republic of Korea.
FAU - Kim, Hyekwon
AU  - Kim H
FAU - Kang, Bokyu
AU  - Kang B
FAU - Yeom, Minjoo
AU  - Yeom M
FAU - Han, Sangyoon
AU  - Han S
FAU - Moon, Hyoungjoon
AU  - Moon H
FAU - Park, Seongjun
AU  - Park S
FAU - Kim, Hyunil
AU  - Kim H
FAU - Song, Daesub
AU  - Song D
FAU - Park, Bongkyun
AU  - Park B
LA  - eng
SI  - GENBANK/GU325635
SI  - GENBANK/GU325636
SI  - GENBANK/GU325637
SI  - GENBANK/GU325638
SI  - GENBANK/GU325639
SI  - GENBANK/GU325640
SI  - GENBANK/GU325641
SI  - GENBANK/GU325642
SI  - GENBANK/GU325643
SI  - GENBANK/GU325644
SI  - GENBANK/GU325645
SI  - GENBANK/GU325646
SI  - GENBANK/GU325647
SI  - GENBANK/GU325648
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Virus Genes
JT  - Virus genes
JID - 8803967
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Animals
MH  - Cluster Analysis
MH  - Genotype
MH  - Molecular Epidemiology
MH  - Molecular Sequence Data
MH  - Open Reading Frames
MH  - *Phylogeny
MH  - Porcine Reproductive and Respiratory Syndrome/*epidemiology/*virology
MH  - Porcine respiratory and reproductive syndrome 
      virus/*classification/genetics/*isolation & purification
MH  - Prevalence
MH  - RNA, Viral/genetics
MH  - Republic of Korea/epidemiology
MH  - Sequence Analysis, DNA
MH  - Swine
EDAT- 2010/01/12 06:00
MHDA- 2010/04/22 06:00
CRDT- 2010/01/12 06:00
PHST- 2009/03/04 00:00 [received]
PHST- 2009/12/08 00:00 [accepted]
PHST- 2010/01/12 06:00 [entrez]
PHST- 2010/01/12 06:00 [pubmed]
PHST- 2010/04/22 06:00 [medline]
AID - 10.1007/s11262-009-0433-3 [doi]
PST - ppublish
SO  - Virus Genes. 2010 Apr;40(2):225-30. doi: 10.1007/s11262-009-0433-3.