PMID- 20063866
OWN - NLM
STAT- MEDLINE
DCOM- 20100518
LR  - 20100129
IS  - 1520-6882 (Electronic)
IS  - 0003-2700 (Linking)
VI  - 82
IP  - 3
DP  - 2010 Feb 1
TI  - Ultrathroughput multiple reaction monitoring mass spectrometry.
PG  - 794-7
LID - 10.1021/ac9026274 [doi]
AB  - A novel transformation of the multiplexing potential to the throughput potential 
      of multiple reaction monitoring mass spectrometry is presented for targeted 
      quantitation of proteins. Herein, this method is termed as ultrathroughput 
      multiple reaction monitoring (UMRM) mass spectrometry. It integrates the use of 
      stable isotope dilution-multiple reaction monitoring mass spectrometry and 
      peptide derivatization with inexpensive and commercial chemicals. One-experiment 
      quantitation of a common signature peptide in 25 different samples demonstrates 
      proof-of-concept for the unprecedented throughput potential of the UMRM 
      technology.
FAU - Yao, Xudong
AU  - Yao X
FAU - Bajrami, Bekim
AU  - Bajrami B
FAU - Shi, Yu
AU  - Shi Y
LA  - eng
PT  - Letter
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
RN  - 0 (Peptides)
RN  - 0 (Proteins)
SB  - IM
MH  - Isotope Labeling
MH  - Mass Spectrometry/*methods
MH  - Peptides/analysis
MH  - Proteins/*analysis
EDAT- 2010/01/13 06:00
MHDA- 2010/05/19 06:00
CRDT- 2010/01/13 06:00
PHST- 2010/01/13 06:00 [entrez]
PHST- 2010/01/13 06:00 [pubmed]
PHST- 2010/05/19 06:00 [medline]
AID - 10.1021/ac9026274 [doi]
PST - ppublish
SO  - Anal Chem. 2010 Feb 1;82(3):794-7. doi: 10.1021/ac9026274.