PMID- 20064268
OWN - NLM
STAT- MEDLINE
DCOM- 20100225
LR  - 20211020
IS  - 1471-2148 (Electronic)
IS  - 1471-2148 (Linking)
VI  - 10
DP  - 2010 Jan 11
TI  - Molecular and cellular evidence for biased mitotic gene conversion in hybrid 
      scallop.
PG  - 6
LID - 10.1186/1471-2148-10-6 [doi]
AB  - BACKGROUND: Concerted evolution has been believed to account for homogenization 
      of genes within multigene families. However, the exact mechanisms involved in the 
      homogenization have been under debate. Use of interspecific hybrid system allows 
      detection of greater level of sequence variation, and therefore, provide 
      advantage for tracing the sequence changes. In this work, we have used an 
      interspecific hybrid system of scallop to study the sequence homogenization 
      processes of rRNA genes. RESULTS: Through the use of a hybrid scallop system 
      (Chlamys farreri female symbol x Argopecten irradians male symbol), here we 
      provide solid molecular and cellular evidence for homogenization of the rDNA 
      sequences into maternal genotypes. The ITS regions of the rDNA of the two scallop 
      species exhibit distinct sequences and thereby restriction fragment length 
      polymorphism (RFLP) patterns, and such a difference was exploited to follow the 
      parental ITS contributions in the F1 hybrid during early development using 
      PCR-RFLP. The representation of the paternal ITS decreased gradually in the 
      hybrid during the development of the hybrid, and almost diminished at the 14th 
      day after fertilization while the representation of the maternal ITS gradually 
      increased. Chromosomal-specific fluorescence in situ hybridization (FISH) 
      analysis in the hybrid revealed the presence of maternal ITS sequences on the 
      paternal ITS-bearing chromosomes, but not vice versa. Sequence analysis of the 
      ITS region in the hybrid not only confirmed the maternally biased conversion, but 
      also allowed the detection of six recombinant variants in the hybrid involving 
      short recombination regions, suggesting that site-specific recombination may be 
      involved in the maternally biased gene conversion. CONCLUSION: Taken together, 
      these molecular and cellular evidences support rapid concerted gene evolution via 
      maternally biased gene conversion. As such a process would lead to the expression 
      of only one parental genotype, and have the opportunities to generate recombinant 
      intermediates; this work may also have implications in novel hybrid zone alleles 
      and genetic imprinting, as well as in concerted gene evolution. In the course of 
      evolution, many species may have evolved involving some levels of hybridization, 
      intra- or interspecific, the sex-biased sequence homogenization could have led to 
      a greater role of one sex than the other in some species.
FAU - Wang, Shi
AU  - Wang S
AD  - Key Laboratory of Marine Genetics and Breeding of Ministry of Education, Ocean 
      University of China, Qingdao 266003, China.
FAU - Zhang, Lingling
AU  - Zhang L
FAU - Hu, Jingjie
AU  - Hu J
FAU - Bao, Zhenmin
AU  - Bao Z
FAU - Liu, Zhanjiang
AU  - Liu Z
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100111
PL  - England
TA  - BMC Evol Biol
JT  - BMC evolutionary biology
JID - 100966975
RN  - 0 (DNA, Ribosomal Spacer)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - DNA, Ribosomal Spacer/genetics
MH  - Female
MH  - *Gene Conversion
MH  - Genotype
MH  - *Hybridization, Genetic
MH  - Male
MH  - Mitosis
MH  - Molecular Sequence Data
MH  - Pectinidae/*genetics
MH  - Polymorphism, Restriction Fragment Length
MH  - Sequence Analysis, DNA
MH  - Species Specificity
PMC - PMC2818637
EDAT- 2010/01/13 06:00
MHDA- 2010/02/26 06:00
PMCR- 2010/01/11
CRDT- 2010/01/13 06:00
PHST- 2009/07/17 00:00 [received]
PHST- 2010/01/11 00:00 [accepted]
PHST- 2010/01/13 06:00 [entrez]
PHST- 2010/01/13 06:00 [pubmed]
PHST- 2010/02/26 06:00 [medline]
PHST- 2010/01/11 00:00 [pmc-release]
AID - 1471-2148-10-6 [pii]
AID - 10.1186/1471-2148-10-6 [doi]
PST - epublish
SO  - BMC Evol Biol. 2010 Jan 11;10:6. doi: 10.1186/1471-2148-10-6.