PMID- 20065299
OWN - NLM
STAT- MEDLINE
DCOM- 20100422
LR  - 20151119
IS  - 1933-7205 (Electronic)
IS  - 1933-7191 (Linking)
VI  - 17
IP  - 3
DP  - 2010 Mar
TI  - Preeclampsia and HELLP syndrome: impaired mitochondrial function in umbilical 
      endothelial cells.
PG  - 219-26
LID - 10.1177/1933719109351597 [doi]
AB  - Preeclampsia (PE) and hemolysis, elevated liver enzymes, and low platelet count 
      (HELLP) syndrome have been linked to congenital fetal disorders of mitochondrial 
      fatty acid oxidation (FAO). Different incidences may argue for the association of 
      noncongenital alterations of mitochondrial energy metabolism with PE/HELLP 
      syndrome. We studied human umbilical vein endothelial cells [HUVEC] as selected 
      part of the feto-placental unit from uncomplicated (n = 46) and diseased (n = 27; 
      17 PE and 10 HELLP) pregnancies by measuring the overall FAO, carnitine 
      palmitoyltransferase 2 (CPT2), respiratory chain (RC) complexes I-V, 
      citratesynthase (CS), lactatedehydrogenase (LDH), hexokinase (HK), 
      phosphofructokinase (PFK), and energy rich phosphates. Maternal and infantile 
      acylcarnitines in blood were investigated post partum. Overall FAO, RC complexes 
      II-V, and CS were significantly compromised in HUVEC from complicated 
      pregnancies; impairment of complexes I + III was not significant. CPT2 and energy 
      charges were unaffected. Lactatedehydrogenase and PFK from complicated 
      pregnancies were upregulated, and HK remained constant. In blood, carnitine was 
      elevated in diseased women and their children, acylcarnitines were higher in 
      affected infants. Impaired mitochondrial function in HUVEC is associated with 
      PE/HELLP syndrome and may be involved in the pathophysiology of these diseases.
FAU - Illsinger, Sabine
AU  - Illsinger S
AD  - Department of Pediatric Kidney, Liver and Metabolic Diseases, Hannover Medical 
      School, Hannover, Germany. illsinger.sabine@mh-hannover.de
FAU - Janzen, Nils
AU  - Janzen N
FAU - Sander, Stefanie
AU  - Sander S
FAU - Schmidt, Karl-Heinz
AU  - Schmidt KH
FAU - Bednarczyk, Jolanthe
AU  - Bednarczyk J
FAU - Mallunat, Lisa
AU  - Mallunat L
FAU - Bode, Julia
AU  - Bode J
FAU - Hagebolling, Friederike
AU  - Hagebolling F
FAU - Hoy, Ludwig
AU  - Hoy L
FAU - Lucke, Thomas
AU  - Lucke T
FAU - Hass, Ralf
AU  - Hass R
FAU - Das, Anibh M
AU  - Das AM
LA  - eng
PT  - Journal Article
DEP - 20100111
PL  - United States
TA  - Reprod Sci
JT  - Reproductive sciences (Thousand Oaks, Calif.)
JID - 101291249
RN  - 0 (Fatty Acids)
RN  - 0 (Multienzyme Complexes)
RN  - 0 (acylcarnitine)
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
RN  - EC 2.3.1.21 (Carnitine O-Palmitoyltransferase)
RN  - EC 2.3.3.1 (Citrate (si)-Synthase)
RN  - EC 2.7.1 - (Phosphofructokinases)
RN  - S7UI8SM58A (Carnitine)
SB  - IM
MH  - Adult
MH  - Carnitine/analogs & derivatives/blood
MH  - Carnitine O-Palmitoyltransferase/metabolism
MH  - Citrate (si)-Synthase/metabolism
MH  - Electron Transport
MH  - Endothelial Cells/enzymology/*metabolism/ultrastructure
MH  - Energy Metabolism
MH  - Fatty Acids/metabolism
MH  - Female
MH  - Glycolysis
MH  - HELLP Syndrome/etiology/*metabolism
MH  - Humans
MH  - Infant, Newborn
MH  - L-Lactate Dehydrogenase/metabolism
MH  - Mitochondria/enzymology/*metabolism
MH  - Mitochondrial Diseases/complications/enzymology/*metabolism
MH  - Multienzyme Complexes/metabolism
MH  - Oxidation-Reduction
MH  - Phosphofructokinases/metabolism
MH  - Pre-Eclampsia/etiology/*metabolism
MH  - Pregnancy
MH  - *Umbilical Veins
EDAT- 2010/01/13 06:00
MHDA- 2010/04/23 06:00
CRDT- 2010/01/13 06:00
PHST- 2010/01/13 06:00 [entrez]
PHST- 2010/01/13 06:00 [pubmed]
PHST- 2010/04/23 06:00 [medline]
AID - 1933719109351597 [pii]
AID - 10.1177/1933719109351597 [doi]
PST - ppublish
SO  - Reprod Sci. 2010 Mar;17(3):219-26. doi: 10.1177/1933719109351597. Epub 2010 Jan 
      11.