PMID- 20067418
OWN - NLM
STAT- MEDLINE
DCOM- 20100406
LR  - 20211020
IS  - 1607-8438 (Electronic)
IS  - 0300-8207 (Print)
IS  - 0300-8207 (Linking)
VI  - 51
IP  - 1
DP  - 2010
TI  - TNF-alpha upregulates expression of BMP-2 and BMP-3 genes in the rat dental 
      follicle--implications for tooth eruption.
PG  - 59-66
LID - 10.3109/03008200903019703 [doi]
AB  - The dental follicle appears to regulate both the alveolar bone resorption and 
      bone formation needed for tooth eruption. Tumor necrosis factor-alpha (TNF-alpha) 
      gene expression is maximally upregulated at postnatal day 9 in the rat dental 
      follicle of the first mandibular molar, a time that correlates with rapid bone 
      growth at the base of the tooth crypt, as well as a minor burst of 
      osteoclastogenesis. TNF-alpha expression is correlated with the expression of 
      bone morphogenetic protein-2 (BMP-2), a molecule expressed in the dental follicle 
      that can promote bone formation. Because BMP-2 signaling may be augmented by bone 
      morphogenetic protein-3 (BMP-3), our objective in this study was to determine if 
      the dental follicle expresses BMP-3 and if TNF-alpha stimulates the dental 
      follicle cells to express BMP-2 and BMP-3. Dental follicles were collected from 
      different postnatal ages of rat pups. Dental follicle cells were incubated with 
      TNF-alpha to study its dosage and time-course effects on gene expression of BMP-2 
      and BMP-3, as determined by real-time RT-PCR. Next, immunostaining was conducted 
      to confirm if the protein was synthesized and ELISA of the conditioned medium was 
      conducted to determine if BMP-2 was secreted. We found that BMP-3 expression is 
      correlated with the expression of TNF-alpha in the dental follicle and TNF-alpha 
      significantly increased BMP-2 and BMP-3 expression in vitro. Immunostaining and 
      ELISA showed that BMP-2 and BMP-3 were synthesized and secreted. This study 
      suggests that TNF-alpha can upregulate the expression of bone formation genes 
      that may be needed for tooth eruption.
FAU - Yao, Shaomian
AU  - Yao S
AD  - Department of Comparative Biomedical Sciences, School of Veterinary Medicine, 
      Louisiana State University, Baton Rouge, Louisiana 70803, USA.
FAU - Prpic, Veronica
AU  - Prpic V
FAU - Pan, Fenghui
AU  - Pan F
FAU - Wise, Gary E
AU  - Wise GE
LA  - eng
GR  - R01 DE008911/DE/NIDCR NIH HHS/United States
GR  - R01 DE008911-20/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PL  - England
TA  - Connect Tissue Res
JT  - Connective tissue research
JID - 0365263
RN  - 0 (Bmp2 protein, rat)
RN  - 0 (Bmp3 protein, rat)
RN  - 0 (Bone Morphogenetic Protein 2)
RN  - 0 (Bone Morphogenetic Protein 3)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Animals
MH  - Bone Development/genetics
MH  - Bone Morphogenetic Protein 2/*genetics
MH  - Bone Morphogenetic Protein 3/*genetics
MH  - Bone Remodeling/genetics
MH  - Dental Sac/cytology/*growth & development/*metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Gene Expression Regulation, Developmental/genetics
MH  - Immunohistochemistry
MH  - Male
MH  - Mandible/cytology/growth & development/metabolism
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Signal Transduction/genetics
MH  - Tooth Eruption/*physiology
MH  - Tumor Necrosis Factor-alpha/*metabolism
MH  - Up-Regulation/genetics
PMC - PMC3104855
MID - NIHMS287545
EDAT- 2010/01/14 06:00
MHDA- 2010/04/07 06:00
PMCR- 2011/05/31
CRDT- 2010/01/14 06:00
PHST- 2010/01/14 06:00 [entrez]
PHST- 2010/01/14 06:00 [pubmed]
PHST- 2010/04/07 06:00 [medline]
PHST- 2011/05/31 00:00 [pmc-release]
AID - 10.3109/03008200903019703 [doi]
PST - ppublish
SO  - Connect Tissue Res. 2010;51(1):59-66. doi: 10.3109/03008200903019703.