PMID- 20067961
OWN - NLM
STAT- MEDLINE
DCOM- 20100721
LR  - 20220331
IS  - 1935-5548 (Electronic)
IS  - 0149-5992 (Print)
IS  - 0149-5992 (Linking)
VI  - 33
IP  - 5
DP  - 2010 May
TI  - Differential effects of cream, glucose, and orange juice on inflammation, 
      endotoxin, and the expression of Toll-like receptor-4 and suppressor of cytokine 
      signaling-3.
PG  - 991-7
LID - 10.2337/dc09-1630 [doi]
AB  - OBJECTIVE: We have recently shown that a high-fat high-carbohydrate (HFHC) meal 
      induces an increase in plasma concentrations of endotoxin (lipopolysaccharide 
      [LPS]) and the expression of Toll-like receptor-4 (TLR-4) and suppresser of 
      cytokine signaling-3 (SOCS3) in mononuclear cells (MNCs) in addition to oxidative 
      stress and cellular inflammation. Saturated fat and carbohydrates, components of 
      the HFHC meal, known to induce oxidative stress and inflammation, also induce an 
      increase in LPS, TLR-4, and SOCS3. RESEARCH DESIGN AND METHODS: Fasting normal 
      subjects were given 300-calorie drinks of either glucose, saturated fat as cream, 
      orange juice, or only water to ingest. Blood samples were obtained at 0, 1, 3, 
      and 5 h for analysis. RESULTS: Indexes of inflammation including nuclear 
      factor-kappaB (NF-kappaB) binding, and the expression of SOCS3, tumor necrosis 
      factor-alpha (TNF-alpha), and interleukin (IL)-1beta in MNCs, increased 
      significantly after glucose and cream intake, but TLR-4 expression and plasma LPS 
      concentrations increased only after cream intake. The intake of orange juice or 
      water did not induce any change in any of the indexes measured. CONCLUSIONS: 
      Although both glucose and cream induce NF-kappaB binding and an increase in the 
      expression of SOCS3, TNF-alpha, and IL-1beta in MNCs, only cream caused an 
      increase in LPS concentration and TLR-4 expression. Equicaloric amounts of orange 
      juice or water did not induce a change in any of these indexes. These changes are 
      relevant to the pathogenesis of atherosclerosis and insulin resistance.
FAU - Deopurkar, Rupali
AU  - Deopurkar R
AD  - Division of Endocrinology, Diabetes, and Metabolism, State University of New York 
      at Buffalo and Kaleida Health, Buffalo, New York, USA.
FAU - Ghanim, Husam
AU  - Ghanim H
FAU - Friedman, Jay
AU  - Friedman J
FAU - Abuaysheh, Sanaa
AU  - Abuaysheh S
FAU - Sia, Chang Ling
AU  - Sia CL
FAU - Mohanty, Priya
AU  - Mohanty P
FAU - Viswanathan, Prabhakar
AU  - Viswanathan P
FAU - Chaudhuri, Ajay
AU  - Chaudhuri A
FAU - Dandona, Paresh
AU  - Dandona P
LA  - eng
GR  - R01 DK069805/DK/NIDDK NIH HHS/United States
GR  - R01 DK075877/DK/NIDDK NIH HHS/United States
GR  - R01-DK-075877/DK/NIDDK NIH HHS/United States
GR  - R01-DK-069805/DK/NIDDK NIH HHS/United States
PT  - Clinical Trial
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20100112
PL  - United States
TA  - Diabetes Care
JT  - Diabetes care
JID - 7805975
RN  - 0 (Acute-Phase Proteins)
RN  - 0 (Blood Glucose)
RN  - 0 (Carrier Proteins)
RN  - 0 (Cytokines)
RN  - 0 (Dietary Carbohydrates)
RN  - 0 (Dietary Fats)
RN  - 0 (Insulin)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (NF-kappa B)
RN  - 0 (RNA, Messenger)
RN  - 0 (SOCS3 protein, human)
RN  - 0 (Suppressor of Cytokine Signaling 3 Protein)
RN  - 0 (Suppressor of Cytokine Signaling Proteins)
RN  - 0 (TLR4 protein, human)
RN  - 0 (Toll-Like Receptor 4)
RN  - 0 (lipopolysaccharide-binding protein)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Acute-Phase Proteins
MH  - Adult
MH  - Animals
MH  - Blood Glucose/metabolism
MH  - Carrier Proteins/blood
MH  - *Citrus sinensis
MH  - Cytokines/genetics/metabolism
MH  - Dietary Carbohydrates/administration & dosage/blood
MH  - Dietary Fats/administration & dosage/blood
MH  - Glucose/*administration & dosage
MH  - Humans
MH  - Inflammation/*blood/immunology
MH  - Insulin/blood
MH  - Lipopolysaccharides/blood
MH  - Membrane Glycoproteins/blood
MH  - Middle Aged
MH  - *Milk
MH  - NF-kappa B/metabolism
MH  - Oxidative Stress/drug effects/immunology
MH  - Postprandial Period/immunology
MH  - RNA, Messenger/metabolism
MH  - Reference Values
MH  - Signal Transduction/drug effects/immunology
MH  - Suppressor of Cytokine Signaling 3 Protein
MH  - Suppressor of Cytokine Signaling Proteins/genetics/*metabolism
MH  - Toll-Like Receptor 4/genetics/*metabolism
PMC - PMC2858203
EDAT- 2010/01/14 06:00
MHDA- 2010/07/22 06:00
PMCR- 2011/05/01
CRDT- 2010/01/14 06:00
PHST- 2010/01/14 06:00 [entrez]
PHST- 2010/01/14 06:00 [pubmed]
PHST- 2010/07/22 06:00 [medline]
PHST- 2011/05/01 00:00 [pmc-release]
AID - dc09-1630 [pii]
AID - 1630 [pii]
AID - 10.2337/dc09-1630 [doi]
PST - ppublish
SO  - Diabetes Care. 2010 May;33(5):991-7. doi: 10.2337/dc09-1630. Epub 2010 Jan 12.