PMID- 20068387
OWN - NLM
STAT- MEDLINE
DCOM- 20100510
LR  - 20200930
IS  - 1555-8576 (Electronic)
IS  - 1538-4047 (Linking)
VI  - 8
IP  - 22
DP  - 2009 Nov
TI  - Detection of deletions and/or amplifications of genes related with lung cancer by 
      multiplex ligation-dependent probe amplification (MLPA) technique.
PG  - 2160-5
AB  - BACKGROUND: Lung cancer has the leading mortality rate among all cancers and it 
      is the second most common cause of death following cardiovascular diseases.The 
      aim of the study was determining deleted and/or amplified regions of 64 different 
      loci previously associated with lung cancer, by using Multiplex 
      Ligation-dependent Probe Amplification (MLPA). RESULTS: The most frequently seen 
      deletions in lung cancerous tissues were in 2p, 3p, 13q, 17p, 16p and the most 
      frequently seen amplifications were in 17q, 8p and 5q. We observed same deletions 
      in the same regions in normal lung tissues as in cancerous tissues in lower 
      frequencies. Deletions in 5q, 8p, 9q, 10p, 11p. 11q, 12p, 14q, 17q and 21q probe 
      regions were seen especially in cancerous tissues. MATERIALS/METHODS: One hundred 
      non small cell lung cancer (NSCLC) tissue samples which had been previously 
      examined histopathologically were included in this investigation. DNA extracts of 
      normal lung tissues from the same patients were used as control group in the 
      study. CONCLUSIONS: As a conclusion, it was determined that MLPA is an 
      alternative technique which can give cheap, fast and reliable results in the 
      screening of lung cancers. The findings obtained in the study are compatible with 
      the literature. MLPA is one of the most important molecular techniques which have 
      been developed recently and it can be used in cancer screening easily and 
      reliably.
FAU - Tepeli, Emre
AU  - Tepeli E
AD  - Eskisehir Osmangazi University School of Medicine Department of Medical Genetics, 
      Eskisehir, Turkey. emre_tepeli@yahoo.com
FAU - Muslumanoglu, Muhammed Hamza
AU  - Muslumanoglu MH
FAU - Uludag, Ahmet
AU  - Uludag A
FAU - Buyukpinarbasili, Nur
AU  - Buyukpinarbasili N
FAU - Ozdemir, Muhsin
AU  - Ozdemir M
FAU - Oznur, Murat
AU  - Oznur M
FAU - Aslan, Huseyin
AU  - Aslan H
FAU - Artan, Sevilhan
AU  - Artan S
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Biol Ther
JT  - Cancer biology & therapy
JID - 101137842
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - Carcinoma, Non-Small-Cell Lung/*genetics/pathology
MH  - Chromosomes, Human/genetics/ultrastructure
MH  - DNA, Neoplasm/genetics
MH  - Female
MH  - *Gene Amplification
MH  - *Gene Deletion
MH  - *Genes, Neoplasm
MH  - Genetic Association Studies
MH  - Genomic Instability
MH  - Humans
MH  - Lung Neoplasms/*genetics/pathology
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Staging
MH  - *Nucleic Acid Amplification Techniques
EDAT- 2010/01/14 06:00
MHDA- 2010/05/11 06:00
CRDT- 2010/01/14 06:00
PHST- 2010/01/14 06:00 [entrez]
PHST- 2010/01/14 06:00 [pubmed]
PHST- 2010/05/11 06:00 [medline]
AID - 9840 [pii]
AID - 10.4161/cbt.8.22.9840 [doi]
PST - ppublish
SO  - Cancer Biol Ther. 2009 Nov;8(22):2160-5. doi: 10.4161/cbt.8.22.9840.