PMID- 20069129
OWN - NLM
STAT- MEDLINE
DCOM- 20100617
LR  - 20211020
IS  - 1110-7251 (Electronic)
IS  - 1110-7243 (Print)
IS  - 1110-7243 (Linking)
VI  - 2009
DP  - 2009
TI  - Reduction of monocyte chemoattractant protein-1 and interleukin-8 levels by 
      ticlopidine in TNF-alpha stimulated human umbilical vein endothelial cells.
PG  - 917837
LID - 10.1155/2009/917837 [doi]
LID - 917837
AB  - Atherosclerosis and its associated complications represent major causes of 
      morbidity and mortality in the industrialized or Western countries. Monocyte 
      chemoattractant protein-1 (MCP-1) is critical for the initiating and developing 
      of atherosclerotic lesions. Interleukin-8 (IL-8), a CXC chemokine, stimulates 
      neutrophil chemotaxis. Ticlopidine is one of the antiplatelet drugs used to 
      prevent thrombus formation relevant to the pathophysiology of atherothrombosis. 
      In this study, we found that ticlopidine dose-dependently decreased the mRNA and 
      protein levels of TNF-alpha-stimulated MCP-1, IL-8, and vascular cell adhesion 
      molecule-1 (VCAM-1) in human umbilical vein endothelial cells (HUVECs). 
      Ticlopidine declined U937 cells adhesion and chemotaxis as compared to TNF-alpha 
      stimulated alone. Furthermore, the inhibitory effects were neither due to 
      decreased HUVEC viability, nor through NF-kB inhibition. These results suggest 
      that ticlopidine decreased TNF-alpha induced MCP-1, IL-8, and VCAM-1 levels in 
      HUVECs, and monocyte adhesion. Therefore, the data provide additional therapeutic 
      machinery of ticlopidine in treatment and prevention of atherosclerosis.
FAU - Hu, Chaur-Jong
AU  - Hu CJ
AD  - Department of Neurology, Taipei Medical University, Taipei 110, Taiwan.
FAU - Lee, Yueh-Lun
AU  - Lee YL
FAU - Shih, Neng-Yao
AU  - Shih NY
FAU - Yang, Yi-Yuan
AU  - Yang YY
FAU - Charoenfuprasert, Suparat
AU  - Charoenfuprasert S
FAU - Dai, Yu-Shan
AU  - Dai YS
FAU - Chang, Su-Mei
AU  - Chang SM
FAU - Tsai, Yu-Hui
AU  - Tsai YH
FAU - Tseng, How
AU  - Tseng H
FAU - Liu, Chia-Yu
AU  - Liu CY
FAU - Leu, Sy-Jye
AU  - Leu SJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100104
PL  - United States
TA  - J Biomed Biotechnol
JT  - Journal of biomedicine & biotechnology
JID - 101135740
RN  - 0 (Chemokine CCL2)
RN  - 0 (Interleukin-8)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transcription Factor RelA)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Vascular Cell Adhesion Molecule-1)
RN  - OM90ZUW7M1 (Ticlopidine)
SB  - IM
MH  - Cell Adhesion/drug effects
MH  - Cell Line
MH  - Chemokine CCL2/genetics/*metabolism
MH  - Chemotaxis/drug effects
MH  - Endothelial Cells/cytology/*drug effects/*metabolism
MH  - Gene Expression Regulation/drug effects
MH  - Humans
MH  - Interleukin-8/genetics/*metabolism
MH  - Protein Transport/drug effects
MH  - RNA, Messenger/genetics/metabolism
MH  - Ticlopidine/*pharmacology
MH  - Transcription Factor RelA/metabolism
MH  - Tumor Necrosis Factor-alpha/*pharmacology
MH  - Umbilical Veins/*cytology
MH  - Vascular Cell Adhesion Molecule-1/genetics/metabolism
PMC - PMC2804117
EDAT- 2010/01/14 06:00
MHDA- 2010/06/18 06:00
PMCR- 2010/01/04
CRDT- 2010/01/14 06:00
PHST- 2009/02/09 00:00 [received]
PHST- 2009/09/02 00:00 [revised]
PHST- 2009/10/08 00:00 [accepted]
PHST- 2010/01/14 06:00 [entrez]
PHST- 2010/01/14 06:00 [pubmed]
PHST- 2010/06/18 06:00 [medline]
PHST- 2010/01/04 00:00 [pmc-release]
AID - 10.1155/2009/917837 [doi]
PST - ppublish
SO  - J Biomed Biotechnol. 2009;2009:917837. doi: 10.1155/2009/917837. Epub 2010 Jan 4.