PMID- 20069613
OWN - NLM
STAT- MEDLINE
DCOM- 20100519
LR  - 20100304
IS  - 1099-1654 (Electronic)
IS  - 1052-9276 (Linking)
VI  - 20
IP  - 2
DP  - 2010 Mar
TI  - Hepatitis C virus nonstructural protein 4B: a journey into unexplored territory.
PG  - 117-29
LID - 10.1002/rmv.640 [doi]
AB  - Hepatitis C virus (HCV) is a positive-strand RNA virus that replicates its genome 
      in a membrane-associated replication complex. Nonstructural protein 4B (NS4B) 
      induces the specific membrane alteration, designated as membranous web (MW), that 
      harbours this complex. HCV NS4B is an integral membrane protein predicted to 
      comprise four transmembrane segments in its central part. The N-terminal part 
      comprises two amphipathic alpha-helices of which the second has the potential to 
      traverse the membrane bilayer, likely upon oligomerisation. The C-terminal part 
      comprises a predicted highly conserved alpha-helix, a membrane-associated 
      amphipathic alpha-helix and two reported palmitoylation sites. NS4B interacts 
      with other viral nonstructural proteins and has been reported to bind viral RNA. 
      In addition, it was found to harbour an NTPase activity. Finally, NS4B has 
      recently been found to have a role in viral assembly. Much work needs to be done 
      with respect to further dissecting these multiple functions as well as providing 
      a refined membrane topology and complete structure of NS4B. Progress in this 
      direction should yield important insights into the functional architecture of the 
      HCV replication complex and may reveal new opportunities for antiviral 
      intervention against a leading cause of chronic hepatitis, liver cirrhosis and 
      hepatocellular carcinoma worldwide.
CI  - (c) 2010 John Wiley & Sons, Ltd.
FAU - Gouttenoire, Jerome
AU  - Gouttenoire J
AD  - Division of Gastroenterology and Hepatology, Centre Hospitalier Universitaire 
      Vaudois, University of Lausanne, Lausanne, Switzerland.
FAU - Penin, Francois
AU  - Penin F
FAU - Moradpour, Darius
AU  - Moradpour D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
PL  - England
TA  - Rev Med Virol
JT  - Reviews in medical virology
JID - 9112448
RN  - 0 (Membrane Proteins)
RN  - 0 (NS4B protein, flavivirus)
RN  - 0 (RNA, Viral)
RN  - 0 (Viral Nonstructural Proteins)
SB  - IM
MH  - Hepacivirus/*physiology
MH  - Membrane Proteins/chemistry/physiology
MH  - Models, Biological
MH  - Protein Binding
MH  - Protein Processing, Post-Translational
MH  - Protein Structure, Tertiary
MH  - RNA, Viral/metabolism
MH  - Viral Nonstructural Proteins/chemistry/*physiology
MH  - *Virus Assembly
MH  - *Virus Replication
RF  - 120
EDAT- 2010/01/14 06:00
MHDA- 2010/05/21 06:00
CRDT- 2010/01/14 06:00
PHST- 2010/01/14 06:00 [entrez]
PHST- 2010/01/14 06:00 [pubmed]
PHST- 2010/05/21 06:00 [medline]
AID - 10.1002/rmv.640 [doi]
PST - ppublish
SO  - Rev Med Virol. 2010 Mar;20(2):117-29. doi: 10.1002/rmv.640.