PMID- 20070186
OWN - NLM
STAT- MEDLINE
DCOM- 20100826
LR  - 20220410
IS  - 1937-335X (Electronic)
IS  - 1937-3341 (Linking)
VI  - 16
IP  - 6
DP  - 2010 Jun
TI  - Osteogenic differentiation of human umbilical cord mesenchymal stromal cells in 
      polyglycolic acid scaffolds.
PG  - 1937-48
LID - 10.1089/ten.TEA.2009.0706 [doi]
AB  - Although human umbilical cord mesenchymal stromal cells (hUCMSCs) have been shown 
      to differentiate along an osteogenic lineage in monolayer culture, the potential 
      of these cells has seldom before been investigated in three-dimensional scaffolds 
      for bone tissue engineering applications. In this 6-week study, we observed 
      osteogenic differentiation of hUCMSCs on polyglycolic acid (PGA) nonwoven mesh 
      scaffolds, and compared seeding densities for potential use in bone tissue 
      engineering. Cells were seeded into PGA meshes with densities of 5, 25, or 50 x 
      10(6) cells/mL scaffold and then cultured in osteogenic medium. Cell 
      proliferation, osteogenic differentiation, and matrix formation were evaluated at 
      weeks 0, 3, and 6. Osteogenic differentiation was observed based on positive 
      alkaline phosphatase activity and an increase of collagen production and calcium 
      incorporation into the extracellular matrix, which increased with higher cell 
      density. During differentiation, runt-related transcription factor (RUNX2), type 
      I collagen (CI), and osteocalcin (OCN) gene expression levels were also 
      increased. In conclusion, exposed to osteogenic signals, hUCMSCs differentiated 
      along an osteogenic lineage as determined by expression of osteogenic markers and 
      matrix formation, and increasing the density of hUCMSCs seeded onto 
      three-dimensional PGA scaffolds led to better osteogenic differentiation.
FAU - Wang, Limin
AU  - Wang L
AD  - Department of Biomedical Engineering, University of Michigan, Ann Arbor, 
      Michigan, USA.
FAU - Dormer, Nathan H
AU  - Dormer NH
FAU - Bonewald, Lynda F
AU  - Bonewald LF
FAU - Detamore, Michael S
AU  - Detamore MS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Tissue Eng Part A
JT  - Tissue engineering. Part A
JID - 101466659
RN  - 0 (Collagen Type I)
RN  - 0 (Core Binding Factor Alpha 1 Subunit)
RN  - 104982-03-8 (Osteocalcin)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Calcium/metabolism
MH  - Cell Differentiation/drug effects
MH  - Cells, Cultured
MH  - Collagen Type I/metabolism
MH  - Core Binding Factor Alpha 1 Subunit/metabolism
MH  - Humans
MH  - Osteocalcin/metabolism
MH  - Osteogenesis/*drug effects
MH  - Polyglycolic Acid/*chemistry/*pharmacology
MH  - Stromal Cells/*cytology/drug effects/metabolism
MH  - Tissue Engineering/*methods
MH  - Tissue Scaffolds/*chemistry
MH  - Umbilical Cord/*cytology
EDAT- 2010/01/15 06:00
MHDA- 2010/08/27 06:00
CRDT- 2010/01/15 06:00
PHST- 2010/01/15 06:00 [entrez]
PHST- 2010/01/15 06:00 [pubmed]
PHST- 2010/08/27 06:00 [medline]
AID - 10.1089/ten.TEA.2009.0706 [doi]
PST - ppublish
SO  - Tissue Eng Part A. 2010 Jun;16(6):1937-48. doi: 10.1089/ten.TEA.2009.0706.