PMID- 20070601
OWN - NLM
STAT- MEDLINE
DCOM- 20100618
LR  - 20111117
IS  - 1399-0039 (Electronic)
IS  - 0001-2815 (Linking)
VI  - 75
IP  - 3
DP  - 2010 Mar
TI  - HLA-DP antibodies before and after renal transplantation.
PG  - 278-85
LID - 10.1111/j.1399-0039.2009.01428.x [doi]
AB  - Human leukocyte antigen (HLA)-DP is considered a target for humoral immune 
      response in clinical transplantation. This study analyses the incidence of HLA-DP 
      antibodies in renal patients. Development and epitope specificity of 
      donor-specific antibodies (DSA) and non-DSA (NDSA) were examined. Pre- and 
      posttransplant sera of 338 patients were screened for HLA-DP antibodies using the 
      luminex single antigen assay. Positive patients, partners and/or kidney donors 
      were HLA-DP typed by sequence-specific oligonucleotides. Potential epitopes were 
      mapped by comparing the amino acid sequences of HLA-DP hypervariable regions 
      (HVR) A-F of recipient, partner and/or donor. Specificities in the sera were 
      aligned to deduce the HVR motif responsible for the antibodies. HLA-DP antibodies 
      were detected in 14% of the patients (48/338). Before transplantation, the 
      antibodies were shown in 23% (10 females and 1 male) and 77% were found after 
      transplantation (30 in patients after the first, 7 after the second graft). 
      Specificities were never restricted to individual mismatched antigens; broad 
      HLA-DP sensitization was found as a rule. A single HVR mismatch was present in 
      80% of the DSA and in 79% of the NDSA. No HLA-DPA specific antibodies were found. 
      Our findings confirm that HLA-DP antibodies are specific for epitopes shared by 
      different HLA-DP antigens, indicating that only a restricted number of mismatched 
      epitopes are recognized by the recipients immune system. Matching for immunogenic 
      HLA-DP epitopes for renal transplantation seems to be functionally more relevant 
      than classical matching at the allelic level.
FAU - Billen, E V A
AU  - Billen EV
AD  - Transplantation Immunology, Tissue Typing Laboratory, Maastricht University 
      Medical Centre, Maastricht, The Netherlands.
FAU - Christiaans, M H L
AU  - Christiaans MH
FAU - Doxiadis, I I N
AU  - Doxiadis II
FAU - Voorter, C E M
AU  - Voorter CE
FAU - van den Berg-Loonen, E M
AU  - van den Berg-Loonen EM
LA  - eng
PT  - Journal Article
DEP - 20100111
PL  - England
TA  - Tissue Antigens
JT  - Tissue antigens
JID - 0331072
RN  - 0 (Antibodies)
RN  - 0 (Complementarity Determining Regions)
RN  - 0 (Epitopes)
RN  - 0 (HLA-DP Antigens)
RN  - 0 (HLA-DP alpha-Chains)
RN  - 0 (HLA-DPA antigen)
SB  - IM
MH  - Antibodies/*immunology
MH  - Antibody Formation/immunology
MH  - Case-Control Studies
MH  - Complementarity Determining Regions/immunology
MH  - Epitopes/analysis/immunology
MH  - Female
MH  - HLA-DP Antigens/*immunology
MH  - HLA-DP alpha-Chains
MH  - Humans
MH  - Kidney Transplantation/*immunology
MH  - Male
MH  - Tissue Donors
EDAT- 2010/01/15 06:00
MHDA- 2010/06/19 06:00
CRDT- 2010/01/15 06:00
PHST- 2010/01/15 06:00 [entrez]
PHST- 2010/01/15 06:00 [pubmed]
PHST- 2010/06/19 06:00 [medline]
AID - TAN1428 [pii]
AID - 10.1111/j.1399-0039.2009.01428.x [doi]
PST - ppublish
SO  - Tissue Antigens. 2010 Mar;75(3):278-85. doi: 10.1111/j.1399-0039.2009.01428.x. 
      Epub 2010 Jan 11.