PMID- 20071840
OWN - NLM
STAT- MEDLINE
DCOM- 20100517
LR  - 20191111
IS  - 1812-2078 (Electronic)
IS  - 1812-2027 (Linking)
VI  - 7
IP  - 26
DP  - 2009 Apr-Jun
TI  - Tearfilm immunoglobulin E (IgE) level in vernal keratoconjunctivitis by ELISA.
PG  - 104-8
AB  - BACKGROUND: Vernal keratoconjunctivitis (VKC) is recurrent chronic allergic 
      conjunctivitis occurring in the prepubertal age-group with secondary involvement 
      of the cornea and is self-limiting in character. The disease is prevalent 
      worldwide but it shows predominance in the areas with dry and warm climate 
      including the South Asia. VKC represents about 3% of the serious ophthalmic 
      disease in some parts of the world where the prevalence is rate is high.Type I 
      hypersensitivity reaction which is IgE-dependent and type IV hypersensitivity 
      reaction have been implicated for the pathogenesis VKC. OBJECTIVE: To determine 
      level of immunoglobulin E (IgE) in the tear fi lm of patients with Vernal 
      Keratoconjunctivitis (VKC) attending outpatient department of BP Koirala Lions 
      Centre for Ophthalmic Studies (BPKLCOS). MATERIALS AND METHODS: Thirty-four VKC 
      patients and thirty-four controls were included in this study. Tear samples were 
      collected using micro- capillary glass tube method and tear IgE levels were 
      measured using an enzyme - linked immunosorbent assay (ELISA). RESULTS: There was 
      high concentration of tear IgE level in VKC (95.09IU/ml) than in controls 
      (1.63IU/ml) though the difference was not statistically significant (p=0.16). No 
      statistically significant difference was observed in male and female gender 
      within VKC group and when compared with control group (in male group, p=0.21 and 
      in female group, p=0.26). There was no statistically significant difference 
      observed in tear IgE level in different age groups within VKC group and when 
      compared with control group (p=0.30). The result did not show any significant 
      difference in tear IgE level with respect to the duration of the disease 
      (p=0.23).There was no statistically significant difference in tear IgE level with 
      different episodes of VKC (p=0.69). No statistically significant difference of 
      IgE concentration in tear was seen among different types of VKC (p=0.53) and 
      grades of tarsal and limbal papillae (p=0.72). CONCLUSION: There was high 
      concentration of tear IgE level observed in VKC.
FAU - Pokharel, S
AU  - Pokharel S
AD  - Ophthalmology Department, Kathmandu Medical College, Sinamangal, Nepal. 
      supranp@yahoo.com
FAU - Shah, D N
AU  - Shah DN
FAU - Joshi, S N
AU  - Joshi SN
FAU - Choudhary, M
AU  - Choudhary M
LA  - eng
PT  - Journal Article
PL  - Nepal
TA  - Kathmandu Univ Med J (KUMJ)
JT  - Kathmandu University medical journal (KUMJ)
JID - 101215359
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Case-Control Studies
MH  - Child
MH  - Child, Preschool
MH  - Conjunctivitis, Allergic/*immunology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Humans
MH  - Immunoglobulin E/*analysis
MH  - Male
MH  - Tears/*immunology
MH  - Young Adult
EDAT- 2010/01/15 06:00
MHDA- 2010/05/18 06:00
CRDT- 2010/01/15 06:00
PHST- 2010/01/15 06:00 [entrez]
PHST- 2010/01/15 06:00 [pubmed]
PHST- 2010/05/18 06:00 [medline]
AID - 10.3126/kumj.v7i2.2700 [doi]
PST - ppublish
SO  - Kathmandu Univ Med J (KUMJ). 2009 Apr-Jun;7(26):104-8. doi: 
      10.3126/kumj.v7i2.2700.