PMID- 20072948
OWN - NLM
STAT- MEDLINE
DCOM- 20100429
LR  - 20191210
IS  - 1233-9687 (Print)
IS  - 1233-9687 (Linking)
VI  - 60
IP  - 4
DP  - 2009
TI  - Differential diagnosis of small round cell tumours (SRCT), fluorescence in situ 
      hybridization (FISH) and immunohistochemical (IHC) study.
PG  - 151-62
AB  - INTRODUCTION: Small round cell tumours (SRCT) of bone and soft tissue constitute 
      a heterogeneous group of neoplasms with similar histological and cytological 
      features. Immunohistochemical studies with panels of antibodies are necessary in 
      order to make the diagnosis. A molecular testing is helpful in many cases. AIM OF 
      THE STUDY: To assess the value of IHC and FISH tests in the differential 
      diagnosis of SRCT. MATERIAL AND METHODS: The material was obtained from patients 
      diagnosed and treated at the Maria Sklodowska-Curie Memorial Cancer 
      Center-Institute in Warsaw between February 2003 and March 2009. One hundred and 
      thirty one patients with the initial diagnosis of SRCT of bone or soft tissue 
      were qualified to the investigation. The material from the primary tumour was 
      obtained by an open or core biopsy in all the patients. During the treatment the 
      patients were monitored, the local recurrence and the distant metastases were 
      reported. The IHC study was performed routinely using wide panels of antibodies. 
      FISH tests: EWSR1, SS18 (SYT), FKHR (F0X01A) and FUS were carried out using dual 
      colour, break-apart probes. RESULTS: IHC tests for CD99 and FLI-1 showed low 
      specificity, had low sensitivity, myogenin staining revealed high specificity and 
      sensitivity. A "lymphoma" panel with LCA, CD20, CD79a, TdT, CD3 showed acceptable 
      specificity and sensitivity. There were 28 (21.37%) uninformative FISH results 
      showing no acceptable signals. CONCLUSIONS: Diagnostic assessment of SRCT 
      requires IHC studies as an introductory method. FISH is necessary in many cases 
      of SRCT for the final diagnosis but it requires well-fixed and processed tissue, 
      otherwise there is a high percentage of uninformative results. A diagnostic 
      algorithm including IHC and FISH tests has been proposed.
FAU - Ptaszynski, Konrad
AU  - Ptaszynski K
AD  - Department of Pathology, Maria Sklodowska-Curie Memorial Cancer Center and 
      Institute of Oncology, Warsaw.
FAU - Szumera-Cieckiewicz, Anna
AU  - Szumera-Cieckiewicz A
FAU - Pekul, Monika
AU  - Pekul M
FAU - Nowecki, Zbigniew
AU  - Nowecki Z
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PL  - Poland
TA  - Pol J Pathol
JT  - Polish journal of pathology : official journal of the Polish Society of 
      Pathologists
JID - 9437432
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Algorithms
MH  - Bone Neoplasms/*diagnosis/metabolism
MH  - Carcinoma, Small Cell/*diagnosis/metabolism
MH  - Diagnosis, Differential
MH  - Female
MH  - Humans
MH  - Immunohistochemistry/*methods
MH  - *In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Retrospective Studies
MH  - Sensitivity and Specificity
MH  - Soft Tissue Neoplasms/*diagnosis/metabolism
MH  - Young Adult
EDAT- 2010/01/15 06:00
MHDA- 2010/04/30 06:00
CRDT- 2010/01/15 06:00
PHST- 2010/01/15 06:00 [entrez]
PHST- 2010/01/15 06:00 [pubmed]
PHST- 2010/04/30 06:00 [medline]
AID - 14003 [pii]
PST - ppublish
SO  - Pol J Pathol. 2009;60(4):151-62.