PMID- 20074241
OWN - NLM
STAT- MEDLINE
DCOM- 20100723
LR  - 20181201
IS  - 1600-0501 (Electronic)
IS  - 0905-7161 (Linking)
VI  - 21
IP  - 3
DP  - 2010 Mar
TI  - Modulation of platelet activation and initial cytokine release by alloplastic 
      bone substitute materials.
PG  - 336-45
LID - 10.1111/j.1600-0501.2009.01830.x [doi]
AB  - OBJECTIVES: Platelet-derived cytokines play a crucial role in tissue 
      regeneration. In regenerative dental medicine, bone substitute materials (BSM) 
      are widely used. However, initial interactions of BSM and platelets are still 
      unknown. The aim of this study was to evaluate the potential of platelet 
      activation and subsequent initial cytokine release by different commercial 
      alloplastic BSM. MATERIAL AND METHODS: Eight commercial BSM of different origins 
      and chemical compositions (tricalcium phosphate, hydroxyapatite, bioactive glass: 
      SiO(2) and mixtures) were incubated with a platelet concentrate (platelet-rich 
      plasma, PRP) of three healthy volunteers at room temperature for 15 min. Platelet 
      count, aggregation, degranulation (activated surface receptor CD62p) and cytokine 
      release (Platelet-derived growth factor, Vascular endothelial growth factor) into 
      the supernatant were quantified. Highly thrombogenic collagen served as a 
      reference. RESULTS: The investigated PRP samples revealed different activation 
      patterns when incubated with different BSM. In general, SiO(2)-containing BSM 
      resulted in high platelet activation and cytokine release. In detail, pure 
      bioactive glass promoted platelet activation most significantly, followed by 
      hybrid BSM containing lower ratios of SiO(2). Additionally, we found indications 
      of cytokine retention by BSM of large specific surfaces. CONCLUSIONS: Platelet 
      activation as well as consecutive storage and slow release of platelet-derived 
      cytokines are desirable attributes of modern BSM. Within the limits of the study, 
      SiO(2)-containing BSM were identified as promising biomaterials. Further 
      investigations on cytokine adsorption and cytokine release kinetics by the 
      respective BSM have to be conducted.
FAU - Klein, M O
AU  - Klein MO
AD  - Department of Oral and Maxillofacial Surgery, Johannes Gutenberg University, 
      Mainz, Germany. klein@mkg.klinik.uni-mainz.de
FAU - Kammerer, Peer W
AU  - Kammerer PW
FAU - Scholz, Thomas
AU  - Scholz T
FAU - Moergel, Maximilian
AU  - Moergel M
FAU - Kirchmaier, Carl Maximilian
AU  - Kirchmaier CM
FAU - Al-Nawas, Bilal
AU  - Al-Nawas B
LA  - eng
PT  - Journal Article
DEP - 20100113
PL  - Denmark
TA  - Clin Oral Implants Res
JT  - Clinical oral implants research
JID - 9105713
RN  - 0 (Bone Substitutes)
RN  - 0 (Calcium Phosphates)
RN  - 0 (P-Selectin)
RN  - 0 (Platelet-Derived Growth Factor)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 7631-86-9 (Silicon Dioxide)
RN  - 91D9GV0Z28 (Durapatite)
RN  - K4C08XP666 (tricalcium phosphate)
MH  - Blood Platelets/*drug effects
MH  - Bone Substitutes/chemistry/*pharmacology
MH  - Calcium Phosphates
MH  - Cell Degranulation
MH  - Durapatite
MH  - Flow Cytometry
MH  - Humans
MH  - P-Selectin/biosynthesis
MH  - Platelet Activation/*drug effects
MH  - Platelet Aggregation
MH  - Platelet Count
MH  - Platelet-Derived Growth Factor/*metabolism
MH  - Platelet-Rich Plasma
MH  - Silicon Dioxide
MH  - Vascular Endothelial Growth Factor A/*metabolism
EDAT- 2010/01/16 06:00
MHDA- 2010/07/24 06:00
CRDT- 2010/01/16 06:00
PHST- 2010/01/16 06:00 [entrez]
PHST- 2010/01/16 06:00 [pubmed]
PHST- 2010/07/24 06:00 [medline]
AID - CLR1830 [pii]
AID - 10.1111/j.1600-0501.2009.01830.x [doi]
PST - ppublish
SO  - Clin Oral Implants Res. 2010 Mar;21(3):336-45. doi: 
      10.1111/j.1600-0501.2009.01830.x. Epub 2010 Jan 13.