PMID- 20075386
OWN - NLM
STAT- MEDLINE
DCOM- 20100514
LR  - 20140730
IS  - 1535-4970 (Electronic)
IS  - 1073-449X (Linking)
VI  - 181
IP  - 9
DP  - 2010 May 1
TI  - Fibrin resistance to lysis in patients with pulmonary hypertension other than 
      thromboembolic.
PG  - 992-6
LID - 10.1164/rccm.200907-1135OC [doi]
AB  - RATIONALE: Reportedly, fibrin isolated from patients with chronic thromboembolic 
      pulmonary hypertension (CTEPH) is resistant to lysis. Persistence of regions 
      within the fibrin beta chain, which mediate cell signaling and migration, could 
      trigger the organization of pulmonary thromboemboli into chronic intravascular 
      scars. OBJECTIVES: Ascertain whether fibrin resistance to lysis occurs in 
      patients with pulmonary hypertension (PAH) other than CTEPH, and in those with 
      prior pulmonary embolism (PE) and no pulmonary hypertension. METHODS: Fibrinogen 
      was purified from 96 subjects (17 with CTEPH, 14 with PAH, 39 with prior PE, and 
      26 healthy control subjects) and exposed to thrombin to obtain fibrin clots. 
      Plasmin-mediated cleavage of fibrin beta chain was assessed hourly over a 6-hour 
      period by polyacrylamide gel electrophoresis. Fibrin band intensity was measured 
      by densitometry of stained gels. Data were normalized to the band intensity of 
      the undigested protein. MEASUREMENTS AND MAIN RESULTS: By 1 hour of digestion, 
      fibrin band intensity had decreased by a median of 25% (interquartile range 
      [IQR], 20 to 27%) in control subjects, and by 15% (IQR, 11 to 18%) in patients 
      with prior PE (P < 0.0001). The 1-hour median reduction in band intensity was 2% 
      (IQR, 1 to 3%) in CTEPH, and 4% (IQR, 2 to 7%) in PAH (P < 0.0001 vs. control 
      subjects and PE). The decline in fibrin band intensity remained significantly 
      different among the four groups up to 6 hours (P < 0.0001). CONCLUSIONS: Fibrin 
      resistance to lysis occurs in pulmonary hypertension other than CTEPH and, to a 
      smaller extent, in patients with prior PE and no pulmonary hypertension.
FAU - Miniati, Massimo
AU  - Miniati M
AD  - Dipartimento di Area Critica Medico Chirugica, Universita di Firenze, Viale 
      Morgagni 85, 50134 Florence, Italy. massimo.miniati@unifi.it
FAU - Fiorillo, Claudia
AU  - Fiorillo C
FAU - Becatti, Matteo
AU  - Becatti M
FAU - Monti, Simonetta
AU  - Monti S
FAU - Bottai, Matteo
AU  - Bottai M
FAU - Marini, Carlo
AU  - Marini C
FAU - Grifoni, Elisa
AU  - Grifoni E
FAU - Formichi, Bruno
AU  - Formichi B
FAU - Bauleo, Carolina
AU  - Bauleo C
FAU - Arcangeli, Chiara
AU  - Arcangeli C
FAU - Poli, Daniela
AU  - Poli D
FAU - Nassi, Paolo Antonio
AU  - Nassi PA
FAU - Abbate, Rosanna
AU  - Abbate R
FAU - Prisco, Domenico
AU  - Prisco D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100114
PL  - United States
TA  - Am J Respir Crit Care Med
JT  - American journal of respiratory and critical care medicine
JID - 9421642
RN  - 9001-31-4 (Fibrin)
SB  - IM
MH  - Adult
MH  - Blood Protein Electrophoresis
MH  - Echocardiography
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Female
MH  - Fibrin/*physiology
MH  - Fibrinolysis
MH  - Hemodynamics
MH  - Humans
MH  - Hypertension, Pulmonary/*blood/physiopathology
MH  - Male
MH  - Middle Aged
MH  - Pulmonary Embolism/blood
EDAT- 2010/01/16 06:00
MHDA- 2010/05/15 06:00
CRDT- 2010/01/16 06:00
PHST- 2010/01/16 06:00 [entrez]
PHST- 2010/01/16 06:00 [pubmed]
PHST- 2010/05/15 06:00 [medline]
AID - 200907-1135OC [pii]
AID - 10.1164/rccm.200907-1135OC [doi]
PST - ppublish
SO  - Am J Respir Crit Care Med. 2010 May 1;181(9):992-6. doi: 
      10.1164/rccm.200907-1135OC. Epub 2010 Jan 14.